%0 Journal Article
%T Specific modulation of p53 binding to consensus sequence within supercoiled DNA by monoclonal antibodies.
%A Brázda V
%A Paleĉek J
%A Pospísilová S
%A Vojtêsek B
%A Paleĉek E
%J Biochem Biophys Res Commun
%V 267
%N 3
%D Jan 2000 27
%M 10673394
%F 3.322
%R 10.1006/bbrc.1999.2056
%X Monoclonal antibodies (MAbs) were used to investigate the binding of insect cell-expressed, wild-type human p53 protein to the consensus sequence (p53CON) in a 474-bp DNA fragment and to supercoiled (sc) DNAs with and without p53CON. Supershifting of p53-DNA complexes by MAbs in agarose gels was applied to studies of activation of p53 for sequence-specific binding within scDNA. C-terminal specific antibody Bp53-10.1 activated the sequence-specific binding of p53 to p53CON within pPGM1 scDNA but did not influence binding of p53 to pBluescript scDNA (not containing p53CON). Incubation of p53 with DO-1 prior to addition of Bp53-10.1 prevented activation of p53 and induced dissociation of a portion of pPGM1 scDNA from the sequence-specific immune complex; no such dissociation was observed if pPGM1 scDNA was replaced by the 474-bp p53CON-containing DNA fragment.