RESULTS: In four-arm olfactometry assays, alate S. avenae showed aphid density-dependent reduced preference to VOC extracts from T. monococcum MDR045 and MDR049. By contrast, alate S. avenae showed aphid density-dependent increased preference to extracts from aphid-susceptible hexaploid wheat, Triticum aestivum var. Solstice and T. monococcum MDR037. Coupled gas chromatography-electroantennography (GC-EAG), using the antennae of alate S. avenae, located 24 electrophysiologically active compounds across all tested accessions. Synthetic blends created from 21 identified EAG-active compounds confirmed bioactivity of corresponding VOC extracts in four-arm olfactometry assays against alate S. avenae.
CONCLUSIONS: Our data suggest that resistance of T. monococcum MDR045 and MDR049 to S. avenae can be at least partially accounted for by antixenosis through antennal perception of specific repellent VOC blends induced by S. avenae feeding behaviour. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
结果:在四臂嗅觉测定法中,alateS.avenae显示,蚜虫密度依赖性降低了对单球菌MDR045和MDR049的VOC提取物的偏好。相比之下,alateS.avenae显示,对蚜虫易感六倍体小麦提取物的蚜虫密度依赖性增加,普通小麦。冬至和T.monoccumMDR037。气相色谱-电触探联用(GC-EAG),使用alateS.avenae的触角,在所有测试材料中定位了24种电生理活性化合物。由21种鉴定的EAG活性化合物产生的合成混合物在四臂嗅觉测定法中证实了相应的VOC提取物对alateS.avenae的生物活性。
结论:我们的数据表明,单球菌毛虫MDR045和MDR049对S.avenae的抗性至少部分可以由通过触角感知S.avenae摄食行为诱导的特定驱避VOC混合物来解释。©2024作者(S)。害虫管理科学由JohnWiley&SonsLtd代表化学工业学会出版。