Abstract：
BACKGROUND: Due to the increasing presence of insecticide resistance across cereal aphid populations, new aphid management strategies, including the engineering of host resistance to aphids into commercial wheat varieties, are required. Previous studies have identified ancestor wheat, Triticum monococcum accessions MDR045 and MDR049, with resistance against the grain aphid, Sitobion avenae. To test the hypothesis that resistance can be accounted for by antixenosis (reduced attractiveness of host plants) via the release of repellent volatile organic compounds (VOCs), we explored the response of S. avenae to MDR045 and MDR049 following S. avenae herbivory, using behaviour and electrophysiology experiments.
RESULTS: In four-arm olfactometry assays, alate S. avenae showed aphid density-dependent reduced preference to VOC extracts from T. monococcum MDR045 and MDR049. By contrast, alate S. avenae showed aphid density-dependent increased preference to extracts from aphid-susceptible hexaploid wheat, Triticum aestivum var. Solstice and T. monococcum MDR037. Coupled gas chromatography-electroantennography (GC-EAG), using the antennae of alate S. avenae, located 24 electrophysiologically active compounds across all tested accessions. Synthetic blends created from 21 identified EAG-active compounds confirmed bioactivity of corresponding VOC extracts in four-arm olfactometry assays against alate S. avenae.
CONCLUSIONS: Our data suggest that resistance of T. monococcum MDR045 and MDR049 to S. avenae can be at least partially accounted for by antixenosis through antennal perception of specific repellent VOC blends induced by S. avenae feeding behaviour. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
RESULTS: In four-arm olfactometry assays, alate S. avenae showed aphid density-dependent reduced preference to VOC extracts from T. monococcum MDR045 and MDR049. By contrast, alate S. avenae showed aphid density-dependent increased preference to extracts from aphid-susceptible hexaploid wheat, Triticum aestivum var. Solstice and T. monococcum MDR037. Coupled gas chromatography-electroantennography (GC-EAG), using the antennae of alate S. avenae, located 24 electrophysiologically active compounds across all tested accessions. Synthetic blends created from 21 identified EAG-active compounds confirmed bioactivity of corresponding VOC extracts in four-arm olfactometry assays against alate S. avenae.
CONCLUSIONS: Our data suggest that resistance of T. monococcum MDR045 and MDR049 to S. avenae can be at least partially accounted for by antixenosis through antennal perception of specific repellent VOC blends induced by S. avenae feeding behaviour. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
摘要：
背景：由于谷物蚜虫种群中杀虫剂抗性的增加，新的蚜虫管理策略，包括将寄主对蚜虫的抗性改造成商业小麦品种，是必需的。以前的研究已经确定了祖先小麦，小麦品种MDR045和MDR049，对谷物蚜虫具有抗性，Sitobionavae.为了检验这一假设，即通过释放排斥性挥发性有机化合物（VOC）可以通过抗病（降低寄主植物的吸引力）来解释抗性，我们探索了马尾草对马尾草对MDR045和MDR049的反应，使用行为和电生理学实验。
结果：在四臂嗅觉测定法中，alateS.avenae显示，蚜虫密度依赖性降低了对单球菌MDR045和MDR049的VOC提取物的偏好。相比之下,alateS.avenae显示，对蚜虫易感六倍体小麦提取物的蚜虫密度依赖性增加，普通小麦。冬至和T.monoccumMDR037。气相色谱-电触探联用(GC-EAG),使用alateS.avenae的触角，在所有测试材料中定位了24种电生理活性化合物。由21种鉴定的EAG活性化合物产生的合成混合物在四臂嗅觉测定法中证实了相应的VOC提取物对alateS.avenae的生物活性。
结论：我们的数据表明，单球菌毛虫MDR045和MDR049对S.avenae的抗性至少部分可以由通过触角感知S.avenae摄食行为诱导的特定驱避VOC混合物来解释。©2024作者（S）。害虫管理科学由JohnWiley&SonsLtd代表化学工业学会出版。
结果：在四臂嗅觉测定法中，alateS.avenae显示，蚜虫密度依赖性降低了对单球菌MDR045和MDR049的VOC提取物的偏好。相比之下,alateS.avenae显示，对蚜虫易感六倍体小麦提取物的蚜虫密度依赖性增加，普通小麦。冬至和T.monoccumMDR037。气相色谱-电触探联用(GC-EAG),使用alateS.avenae的触角，在所有测试材料中定位了24种电生理活性化合物。由21种鉴定的EAG活性化合物产生的合成混合物在四臂嗅觉测定法中证实了相应的VOC提取物对alateS.avenae的生物活性。
结论：我们的数据表明，单球菌毛虫MDR045和MDR049对S.avenae的抗性至少部分可以由通过触角感知S.avenae摄食行为诱导的特定驱避VOC混合物来解释。©2024作者（S）。害虫管理科学由JohnWiley&SonsLtd代表化学工业学会出版。
