Abstract：
In the previous study (Im et al., 2022), we revealed microplastic (MP) was accumulated and cleared through the kidneys via PET imaging. Here, we aimed to identify the renal dysfunction due to polyethylene (PE) MP in the kidney tissue. Mice were exposed to 100 ppm (∼equivalent to 0.1 mg/mL)/100 μL of PE for 12 weeks (n = 10). PE uptake in the kidney tissues was confirmed using confocal microscopy. QuantSeq analysis was performed to determine gene expression. Renal function assessment was performed using 99mTc-Diethylene triamine penta acetic acid or 99mTc-Dimercaptosuccinic acid. Measurement of creatinine, BUN, and albumin levels in serum and urine samples was also estimated. [18F]-FDG was also acquired. PE increased expression of Myc, CD44, Programmed Death-Ligand 1 (PD-L1), and Hypoxia-Inducible Factor (HIF)-1α, which indicates a potential link to an increased risk of early-onset cancer. An increase in glucose metabolism of [18F]-FDG were observed. We assessed renal failure using 99mTc-Diethylene triamine penta acetic acid and 99mTc-Dimercaptosuccinic acid scintigraphy to determine the renal function. Renal failure was confirmed using serum and urine creatinine, serum blood urea nitrogen levels, serum albumin levels, and urine albumin levels in PE exposed mice, relative to the control. In sum, PE exposure induced renal dysfunction in a murine model.
摘要：
在先前的研究中（Im等人。,2022),我们通过PET成像发现微塑料(MP)通过肾脏积聚和清除.这里,我们旨在确定肾脏组织中聚乙烯(PE)MP所致的肾功能障碍.小鼠暴露于100ppm（~相当于0.1mg/mL）/100μL的PE12周（n=10）。使用共聚焦显微镜确认肾组织中的PE摄取。进行QuantSeq分析以确定基因表达。使用99mTc-二亚乙基三胺五乙酸或99mTc-二巯基琥珀酸进行肾功能评估。肌酐的测量，BUN,并且还估计了血清和尿液样本中的白蛋白水平。还获得了[18F]-FDG。PE增加Myc的表达，CD44，程序性死亡配体1（PD-L1），和缺氧诱导因子（HIF）-1α，这表明了与早发性癌症风险增加的潜在联系。观察到[18F]-FDG的葡萄糖代谢增加。我们使用99mTc-二亚乙基三胺五乙酸和99mTc-二巯基琥珀酸闪烁显像来评估肾功能衰竭，以确定肾功能。使用血清和尿肌酐证实肾衰竭，血清尿素氮水平，血清白蛋白水平，和暴露于PE的小鼠的尿白蛋白水平，相对于控制。总之,PE暴露在小鼠模型中诱导肾功能障碍。
