Abstract：
Dermal papilla cells (DPCs) exhibit self-recovery ability, which may be involved in hair growth. Therefore, we tested whether DPCs subjected to temporary growth-inhibiting stress (testosterone, 17β-estradiol, mitomycin C, or undernutrition) treatments exhibit self-recovery behavior that can activate hair follicle growth, and examined the changes in cell proliferation capacity and gene expression. Related proteins were identified and their relationships with the hair cycle was examined using a mouse model. Recovery-period DPCs (i.e., from day 3 after loading) were subjected to microarray analysis to detect genetic variations common to each stress treatment. Co-culture of recovery-period DPCs and outer root sheath cells (ORSCs) confirmed the promotion of ORSC proliferation, suggesting that the activation of hair follicle growth is promoted via signal transduction. Chitinase 3-like 1 (CHI3L1) and C-X-C motif chemokine 5 (CXCL5) exhibited ORSC proliferation-promoting effects. Measurement of protein content in the skin during each phase of the hair cycle in mice revealed that CHI3L1 and CXCL5 secretion increased immediately after anagen transition. In a hair-loss mouse model treated with testosterone or 17β-estradiol, CHI3L1 and CXCL5 secretion was lower in treated telogen skin than in untreated skin. Our results suggest that CHI3L1 and CXCL5 secreted by recovery-state DPCs promote hair growth.
摘要：
毛乳头细胞（DPC）表现出自我恢复能力，这可能与头发生长有关。因此,我们测试了DPC是否受到暂时的生长抑制压力(睾酮，17β-雌二醇，丝裂霉素C,或营养不良）治疗表现出自我恢复行为，可以激活毛囊生长，并检查了细胞增殖能力和基因表达的变化。鉴定相关蛋白，并使用小鼠模型检查其与毛发周期的关系。恢复期DPC(即，从加载后第3天开始）进行微阵列分析，以检测每种应激治疗常见的遗传变异。恢复期DPCs与外根鞘细胞(ORSCs)共培养证实促进ORSC增殖,提示毛囊生长的激活是通过信号转导促进的。几丁质酶3样1(CHI3L1)和C-X-C基序趋化因子5(CXCL5)显示出促进ORSC增殖的作用。在小鼠毛发周期的每个阶段测量皮肤中的蛋白质含量表明，CHI3L1和CXCL5的分泌在生长期过渡后立即增加。在用睾酮或17β-雌二醇治疗的脱发小鼠模型中，CHI3L1和CXCL5的分泌在处理过的皮肤中低于未处理的皮肤。我们的结果表明，恢复状态DPC分泌的CHI3L1和CXCL5促进头发生长。
