UNASSIGNED: Female Sprague-Dawley rats were randomly divided into 4 groups: a negative control group, a group of healthy rats exposed to BaP, a group of rats with ovalbumin (OVA)-induced AR, and a group of AR model rats exposed to BaP. Nasal symptoms and levels of OVA-specific serum immunoglobulin E (IgE) were measured in each individual rat. Moreover, examination of goblet cell hyperplasia and collagen deposition was carried out with periodic acid-Schiff (PAS) staining and Masson trichrome (MT) staining. Mucin 5AC (MUC5AC) expression was assessed by immunohistochemistry.
UNASSIGNED: BaP significantly increased the number of sneezes, the number of nasal rubs and the levels of OVA-specific serum IgE in rats with AR. Statistically significant differences in goblet cell hyperplasia and collagen deposition were observed between the BaP-exposed AR model group and the AR model group. Immunohistochemical results showed that the nasal mucosa of AR model rats displayed markedly elevated MUC5AC expression after BaP exposure.
UNASSIGNED: Our data indicate that mucus hypersecretion and the development of nasal remodeling might be pathophysiologic mechanisms underlying increased susceptibility to AR after exposure to BaP.
■雌性Sprague-Dawley大鼠随机分为4组:阴性对照组,一组暴露于BaP的健康大鼠,一组卵清蛋白(OVA)诱导的AR大鼠,和一组暴露于BaP的AR模型大鼠。在每只大鼠中测量鼻部症状和OVA特异性血清免疫球蛋白E(IgE)的水平。此外,用高碘酸希夫(PAS)染色和马森三色(MT)染色检查杯状细胞增生和胶原蛋白沉积。通过免疫组织化学评估粘蛋白5AC(MUC5AC)表达。
■BaP显著增加了打喷嚏的次数,AR大鼠的鼻擦次数和OVA特异性血清IgE水平。BaP暴露AR模型组与AR模型组之间杯状细胞增生和胶原沉积差异有统计学意义。免疫组织化学结果显示,BaP暴露后,AR模型大鼠鼻黏膜MUC5AC表达明显升高。
■我们的数据表明,粘液分泌过多和鼻重塑的发展可能是暴露于BaP后对AR易感性增加的病理生理机制。