Abstract：
In the field of tissue engineering, the extracellular matrix (ECM) is considered an important element for promoting neural regeneration after spinal cord injury (SCI). Dental pulp stem cells (DPSCs), mesenchymal stem cells that originate from the neural crest, are easy to harvest and culture in vitro, express a variety of neurotrophic factors (NTFs) and deposit a large amount of ECM, making them a good choice for stem cell- or ECM-based treatment of SCI. In the present study, decellularized extracellular matrix (dECM) derived from DPSC sheets is used for the treatment of SCI. Optimization experiments reveal that incubating DPSC sheets with 1% Triton X-100 for 5 min is the best procedure for preparing DPSC dECM. It is found that DPSC dECM promotes nerve repair and regeneration after SCI and restores hindlimb motor function in rats. Mechanistically, DPSC dECM facilitates the migration and neural differentiation of neural stem cells, as well as M2 polarization of microglia, and inhibits the formation of glial scars. This study suggests that the use of DPSC dECM is a potential strategy for the treatment of SCI.
摘要：
在组织工程领域，细胞外基质（ECM）被认为是促进脊髓损伤（SCI）后神经再生的重要元素。牙髓干细胞（DPSC），源自神经c的间充质干细胞，易于在体外收获和培养，表达多种神经营养因子(NTFs)并沉积大量ECM,使它们成为干细胞或基于ECM的SCI治疗的好选择。在本研究中,衍生自DPSC片的脱细胞细胞外基质(dECM)用于治疗SCI。优化实验表明，将DPSC片与1％TritonX-100孵育5分钟是制备DPSCdECM的最佳程序。发现DPSCdECM促进SCI后神经修复和再生，并恢复大鼠后肢运动功能。机械上,DPSCdECM促进神经干细胞的迁移和神经分化，以及小胶质细胞的M2极化，抑制胶质疤痕的形成.这项研究表明，使用DPSCdECM是治疗SCI的潜在策略。
