Abstract：
The acellular slime mold Physarum polycephalum is a large, unicellular amoeba, which, due to its huge size, is well suited to investigate chemotaxis and cellular locomotion. The myxomycete has an astonishing behavioral repertoire and is highly responsive to changes in its environment, which map to changes in its tubular network, internal cytoplasm flow, and cytoskeleton. The behavioral repertoire includes problem-solving, decision-making, and memory. P. polycephalum\'s chemo- and phototaxis are especially well studied. This chapter describes how to cultivate different morphotypes of P. polycephalum (micro-, meso-, and macroplasmodia). Furthermore, the setup of a chemotaxis experiment and the acquisition and analysis of chemotaxis data is described.
摘要：
无细胞粘液霉菌Physarumpolyphealum是一个很大的，单细胞变形虫,which,由于其巨大的尺寸，非常适合研究趋化性和细胞运动。粘菌具有惊人的行为特征，并且对环境变化具有高度响应，映射到其管状网络的变化，内部细胞质流，和细胞骨架。行为库包括解决问题，决策,和记忆。多脑假单胞菌的趋化和趋光性得到了特别好的研究。本章介绍了如何培养不同形态型的多头假单胞菌(显微，中观-，和大型疟原虫）。此外，描述了趋化性实验的设置以及趋化性数据的获取和分析。
