Abstract:
Oil-in-water emulsions (EM) have been extensively used for the encapsulation of lipophilic bioactive compounds and posterior incorporation into food matrices to obtain functional foods. Conversely, novel excipient oil-in-water emulsions (EXC) present identical composition and structure as EM, albeit are not bioactive by themselves since no bioactive compound is encapsulated. Instead, EXC aims at improving the bioavailability of foods\' natural bioactive compounds upon co-ingestion with nutrient-rich foods. In this work, EM and EXC were produced and their stability and functionality as delivery systems for α-tocopherol compared. Emulsions were formulated with corn oil and lecithin, and their composition was optimized using experimental designs. Formulations produced with 3 % lecithin and 5 % oil attained smallest particles sizes with the lowest polydispersity index of all tested formulations and remained stable up to 60 days. Encapsulation of α-tocopherol did not have a significative impact on the structural properties of the particles produced with the same composition. α-tocopherol stability during in vitro digestion was superior in EM regardless the processing methodology (EM stability < 50 %, EXC stability < 29 %), indicating that EM offered greater protection against the digestive environment. α-tocopherol\'s bioaccessibility was significantly increased when encapsulated or when digested with added excipient emulsions (82-92 % and 87-90 % for EM and EXC, respectively). In conclusion, EM were more efficient vehicles for the selected bioactive compound, however, the good results obtained with EXC imply that excipient emulsions have a great potential for applications on foods to improve their natural bioactive compounds\' bioavailability without the need of further processing.
摘要:
水包油乳液(EM)已被广泛用于包封亲脂性生物活性化合物和随后掺入食品基质中以获得功能性食品。相反,新型赋形剂水包油乳液(EXC)具有与EM相同的组成和结构,尽管本身没有生物活性,因为没有生物活性化合物被包封。相反,EXC旨在提高食品的生物利用度\'天然生物活性化合物与营养丰富的食物共同摄入。在这项工作中,制备了EM和EXC,并比较了它们作为α-生育酚递送系统的稳定性和功能性。用玉米油和卵磷脂配制乳液,并使用实验设计优化了它们的组成。用3%卵磷脂和5%油生产的制剂获得了所有测试制剂的最小粒度和最低多分散指数,并且保持稳定长达60天。α-生育酚的包封对用相同组成制备的颗粒的结构性质没有显著影响。α-生育酚在体外消化过程中的稳定性优于EM,无论处理方法如何(EM稳定性<50%,EXC稳定性<29%),表明EM对消化环境提供了更大的保护。α-生育酚的生物可及性显着增加,当封装或消化与添加赋形剂乳液(82-92%和87-90%的EM和EXC,分别)。总之,EM是所选生物活性化合物的更有效载体,然而,使用EXC获得的良好结果意味着赋形剂乳液在食品上具有巨大的应用潜力,可以提高其天然生物活性化合物的生物利用度,而无需进一步加工。