Abstract：
This study examined the suppressive effects of 16 selected plant-based foods on α-glucosidase and pancreatic lipase and their antioxidant properties. Among these, the bark of Cinnamomum cassia (Cinnamon, WLN-FM 15) showed the highest inhibitory activity against α-glucosidase and the highest antioxidant activity. Additionally, WLN-FM 15 showed promising results in the other tests. To further identify the bioactive constituents of WLN-FM 15, a multi-bioactivity-labeled molecular networking approach was used through a combination of GNPS-based molecular networking, DPPH-HPLC, and affinity-based ultrafiltration-HPLC. A total of nine procyanidins were identified as antioxidants and inhibitors of α-glucosidase and pancreatic lipase in WLN-FM 15. Subsequently, procyanidins A1, A2, B1, and C1 were isolated, and their efficacy was confirmed through functional assays. In summary, WLN-FM 15 has the potential to serve as a functional food ingredient with the procyanidins as its bioactive constituents. These results also suggest that the multi-bioactivity-labeled molecular networking approach is reliable for identifying bioactive constituents in plant-based foods.
摘要：
这项研究检查了16种选定的植物性食品对α-葡萄糖苷酶和胰脂肪酶的抑制作用及其抗氧化性能。其中,肉桂的树皮（肉桂，WLN-FM15）对α-葡萄糖苷酶的抑制活性最高，抗氧化活性最高。此外,WLN-FM15在其他测试中显示有希望的结果。为了进一步确定WLN-FM15的生物活性成分，通过结合基于GNPS的分子网络，使用了一种多生物活性标记的分子网络方法，DPPH-HPLC,和基于亲和力的超滤-HPLC。在WLN-FM15中，共有9种原花青素被鉴定为α-葡萄糖苷酶和胰脂肪酶的抗氧化剂和抑制剂。随后，分离了原花青素A1，A2，B1和C1，其功效通过功能分析得到证实。总之,WLN-FM15具有作为功能性食品成分的潜力，原花青素作为其生物活性成分。这些结果还表明，多生物活性标记的分子网络方法对于识别植物性食品中的生物活性成分是可靠的。
