Abstract：
Hydrogen-rich water (HRW) is a beverage containing a high concentration of hydrogen that has been researched for its antioxidant, anti-apoptotic, and anti-inflammatory properties in asthma. This study investigates the potential therapeutic impact of HRW on the gut-lung axis. Using 16S rRNA and serum metabolomics, we examined changes in gut microbiota and serum metabolites in asthmatic mice after HRW intervention, followed by validation experiments. The findings revealed that HRW influenced gut microbiota by increasing Ligilactobacillus and Bifidobacterium abundance and enhancing the presence of indole-3-acetic acid (IAA), a microbially derived serum metabolite. Both in vivo and in vitro experiments showed that HRW\'s protective effects against airway inflammation in asthmatic mice may be linked to the gut microbiota, with IAA potentially playing a role in reducing asthmatic airway inflammation through the aryl hydrocarbon receptors (AhR) signaling pathway. In summary, HRW can modify gut microbiota, increase Bifidobacterium abundance, elevate microbial-derived IAA levels, and activate AhR, which could potentially alleviate inflammation in asthma.
摘要：
富氢水（HRW）是一种含有高浓度氢气的饮料，已被研究为其抗氧化剂，抗凋亡，和哮喘的抗炎特性。这项研究调查了HRW对肠-肺轴的潜在治疗影响。使用16SrRNA和血清代谢组学，我们检查了HRW干预后哮喘小鼠的肠道菌群和血清代谢物的变化，其次是验证实验。研究结果表明，HRW通过增加双歧杆菌和双歧杆菌的丰度并增强吲哚-3-乙酸（IAA）的存在来影响肠道微生物群，微生物来源的血清代谢物。体内和体外实验均表明，HRW对哮喘小鼠气道炎症的保护作用可能与肠道菌群有关，IAA可能通过芳香烃受体（AhR）信号通路在减少哮喘气道炎症中发挥作用。总之,HRW可以改变肠道微生物群，增加双歧杆菌的丰度，提高微生物来源的IAA水平，激活AhR,这可能会缓解哮喘的炎症。
