Abstract：
Alizarin complexone-modified gold nanoparticles (Au0-NPsALz) were synthesized using a proposed ultrasonic irradiation-assisted chemical reduction method. Ultrasonic irradiation powers, reaction time and alizarin complexone concentration had been proven to be the main parameters for controlling the nucleation and growth of Au0-NPsALz. In the synthesized ultrasonic irradiation-assisted chemical reduction conditions, Au0-NPsALz had a spherical oriented morphology with a uniform size of 17.84 ± 1.37 nm and are shiny red with a surface plasmon resonance (SPR) of 535 nm. A rapid colorimetric and fluorometric dual-mode detection strategy for selective detection of histamine in seafood was developed based on the self-assembly of Au0-NPsALz-Ni (II) complexes. Ni (II) can capture the histamine molecules close to Au0-NPsALz surfaces, making changes in the colorimetric and fluorometric responses of the solution. The quantitative analysis of histamine was realized through the variation of dual-signal colorimetric and fluorometric responses. Such Au0-NPsALz sensor offered good detection sensitivity for histamine with a detection limit (LOD) of 59.32 μmol L-1 and 116.20 μmol L-1 and wide linear response within the range of 10-10000 μmol L-1 (R2 = 0.9952) and 100-5000 μmol L-1 (R2 = 0.9947) for colorimetric and fluorometric measurement, respectively. Recoveries ranging from 94.99 to 103.29 % and 97.67-106.88 % for colorimetric and fluorometric assay were obtained, showing low levels of matrix effects. Particularly, the results of the dual-mode sensor were also validated by comparing with the HPLC method for improving the assay accuracy and dependability. Ultimately, the developed Au0-NPsALz colorimetric and fluorometric probe performs excellently in practical applications, with promising results for detecting histamine in seafood products.
摘要：
使用拟议的超声辐照辅助化学还原法合成了茜素络合物修饰的金纳米颗粒（Au0-NPsALz）。超声波辐照功率,反应时间和茜素络合酮浓度已被证明是控制Au0-NPsALz成核和生长的主要参数。在合成的超声辐照辅助化学还原条件下，Au0-NPsALz具有球形取向的形态,具有17.84±1.37nm的均匀尺寸,并且是有光泽的红色,具有535nm的表面等离子体共振(SPR)。基于Au0-NPsALz-Ni（II）络合物的自组装，开发了一种快速比色和荧光双模式检测策略，用于选择性检测海鲜中的组胺。Ni(II)可以捕获靠近Au0-NPsALz表面的组胺分子，改变溶液的比色和荧光响应。通过双信号比色和荧光响应的变化来实现组胺的定量分析。这种Au0-NPsALz传感器对组胺具有良好的检测灵敏度，检出限（LOD）为59.32μmolL-1和116.20μmolL-1，线性响应范围为10-10000μmolL-1（R2=0.9952）和100-5000μmolL-1（R2=0.9947），用于比色和荧光测量。分别。比色和荧光测定的回收率为94.99至103.29%和97.67-106.88%，显示低水平的基质效应。特别是,通过与HPLC方法的比较，还验证了双模式传感器的结果，以提高测定的准确性和可靠性。最终,开发的Au0-NPsALz比色和荧光探针在实际应用中表现出色，对检测海鲜产品中的组胺有希望的结果。
