Abstract：
Green synthesized iron/manganese nanoparticles (Fe/Mn NPs), acted as an exogenous promoter to enhance the lignin-degrading bacteria Comamonas testosteroni FJ17 resulting in more efficient removal of bisphenol A (BPA). Batch experiments demonstrated that removal efficiency of BPA via cells at a BPA concentration of 10 mg·L-1 increased by 20.9 % when exposed to 100 mg·L-1 Fe/Mn NPs after 48 h (93.63 %) relative to an unexposed control group (72.70 %). TEM and 3D-EEM analysis confirmed that the cell membrane thickness increased from 47 to 80 nm under Fe/Mn NPs exposure, and the TB-EPS secretion was promoted. Meanwhile, Fe/Mn NPs facilitated greater electron transfer capacity of c-cytochrome (0.55 V reduction peak) and an unknown cytochrome substance (0.7 V oxidation peak) on the surface of cells. Studies of the effect of Fe/Mn NPs on both the growth and activity of laccase cells showed that both biomass and laccase secretion increased significantly during the logarithmic growth period (6-36 h). LC-MS analysis and toxicity assessment indicated that Fe/Mn NPs decreased the degradation time of BPA and efficiently reduced the toxicity of its by-products. Transcriptomic analysis revealed 315 up-regulation of the key genes associated with energy supply, membrane translocation, and metabolic pathways upon exposure to Fe/Mn NPs. Such as MFS transporter (2.27-fold), diguanylate cyclase (1.76-fold) and protocatechuate-3,4-dioxygenase (1.62-fold). Overall, Fe/Mn NPs accelerated proliferation by enhancing metabolic capacity and nutrient transport processes, which serves to improve the efficiency of BPA removal.
摘要：
绿色合成铁/锰纳米粒子(Fe/MnNPs),充当外源启动子以增强木质素降解细菌CoamonasteststeroniFJ17，从而更有效地去除双酚A（BPA）。批量实验表明，在BPA浓度为10mg·L-1的情况下，暴露于100mg·L-1Fe/MnNPs48h后，通过细胞对BPA的去除效率提高了20.9％（93.63％），相对于未暴露的对照组（72.70％）。TEM和3D-EEM分析证实，在Fe/MnNPs暴露下，细胞膜厚度从47nm增加到80nm，促进TB-EPS分泌。同时,Fe/MnNP促进了细胞表面c-细胞色素（0.55V还原峰）和未知细胞色素物质（0.7V氧化峰）的更大的电子转移能力。对Fe/MnNP对漆酶细胞生长和活性的影响的研究表明，在对数生长期（6-36h），生物量和漆酶分泌均显着增加。LC-MS分析和毒性评估表明，Fe/MnNPs缩短了BPA的降解时间，并有效降低了其副产物的毒性。转录组分析显示315个与能量供应相关的关键基因上调，膜易位,和暴露于Fe/MnNP时的代谢途径。如MFS运输机（2.27倍），二鸟苷酸环化酶（1.76倍）和原儿茶酸-3,4-双加氧酶（1.62倍）。总的来说,Fe/MnNPs通过增强代谢能力和养分运输过程加速增殖，这有助于提高BPA的去除效率。
