PDF(Pubmed)
Abstract：
In the age of information explosion, the exponential growth of digital data far exceeds the capacity of current mainstream storage media. DNA is emerging as a promising alternative due to its higher storage density, longer retention time, and lower power consumption. To date, commercially mature DNA synthesis and sequencing technologies allow for writing and reading of information on DNA with customization and convenience at the research level. However, under the disconnected and nonspecialized mode, DNA data storage encounters practical challenges, including susceptibility to errors, long storage latency, resource-intensive requirements, and elevated information security risks. Herein, we introduce a platform named DNA-DISK that seamlessly streamlined DNA synthesis, storage, and sequencing on digital microfluidics coupled with a tabletop device for automated end-to-end information storage. The single-nucleotide enzymatic DNA synthesis with biocapping strategy is utilized, offering an ecofriendly and cost-effective approach for data writing. A DNA encapsulation using thermo-responsive agarose is developed for on-chip solidification, not only eliminating data clutter but also preventing DNA degradation. Pyrosequencing is employed for in situ and accurate data reading. As a proof of concept, DNA-DISK successfully stored and retrieved a musical sheet file (228 bits) with lower write-to-read latency (4.4 min of latency per bit) as well as superior automation compared to other platforms, demonstrating its potential to evolve into a DNA Hard Disk Drive in the future.
摘要：
在信息爆炸的时代,数字数据的指数增长远远超过了当前主流存储介质的容量。DNA由于其更高的存储密度而成为一种有前途的替代品，更长的保留时间，和更低的功耗。迄今为止,商业上成熟的DNA合成和测序技术允许在研究水平上定制和方便地写入和读取关于DNA的信息。然而,在断开连接和非专业化模式下，DNA数据存储遇到实际挑战，包括对错误的敏感性，长存储延迟，资源密集型需求，和升高的信息安全风险。在这里，我们引入了一个名为DNA-DISK的平台，它无缝地简化了DNA合成，storage,并在数字微流体上进行测序，再加上桌面设备，用于自动端到端信息存储。利用生物封盖策略的单核苷酸酶促DNA合成，为数据写入提供了一种经济实惠、经济高效的方法。开发了一种使用热响应性琼脂糖进行DNA封装的芯片上固化，不仅消除了数据混乱，还防止了DNA降解。焦磷酸测序用于原位和准确的数据读取。作为概念的证明，与其他平台相比，DNA-DISK成功存储和检索了一个乐谱文件（228位），具有更低的读写延迟（每位延迟4.4分钟）以及出色的自动化能力。展示了其在未来发展成为DNA硬盘驱动器的潜力。
