PDF(Pubmed)
Abstract：
Freshwater crabs (Potamiscus manipuriensis), commonly consumed as local delicacies by the native people in the state of Manipur, were found to harbour metacercariae of Microphallus sp. (Family Microphyllidae), which were morphologically different from metacercariae of Microphallus spp reported earlier from different regions. So, PCR-based molecular characterization of this metacercaria was done utilizing rDNA marker regions: larger subunit (LSU) or 28S (D1-D3 region) and inter-transcribed spacer 2 (ITS2). Sequence and phylogenetic analyses confirmed that the taxon under study belonged to family Microphyllidae of genus Microphallus.
摘要：
淡水蟹（Potamiscusmanipuriensis），通常在曼尼普尔邦被当地人作为当地美食消费，被发现藏有Microphallussp。（微叶科），它们在形态上与早期报道的不同地区的小phi属的后cer虫不同。所以,利用rDNA标记区：较大的亚基（LSU）或28S（D1-D3区）和转录间间隔区2（ITS2）进行了基于PCR的这种后a的分子表征。序列和系统发育分析证实，所研究的分类单元属于小虫属的小虫科。
