PDF(Pubmed)
Abstract：
UNASSIGNED: To validate the feasibility of ultrasound in assessing the curative effect of botulinum toxin type A (BTXA) in treating hypertrophic scar (HS).
UNASSIGNED: Eight healthy New Zealand long-eared rabbits were utilized in the study. Four wounds, each measuring 1.0 cm in diameter, were created on both ears of each rabbit. Immediately after surgery, each of these wounds received an injection containing a distinct concentration of BTXA. On postoperative week 6, scar thickness, vascularity, and hardness were assessed based on high frequency ultrasound (HFUS), superb microvascular imaging (SMI), shear wave elastography (SWE), Masson staining, and immunohistochemical staining for CD31.
UNASSIGNED: All wounds healed well, and HSs formed after 6 weeks post-surgery. Scar thickness based on HFUS presented a significant decrease with increasing BTXA concentration (p < 0.05), aligning with the gross morphology. Simultaneously, scar stiffness, evaluated using SWE, showed a significant decrease in accordance with the variation of the collagen volume fraction, which refers to the ratio of the collagen positive area to the total area (p < 0.05). Although the vascularity index obtained by SMI did not exhibit a statistically significant change across different BTXA concentrations, this technique effectively illustrated the microvascular perfusion in HS. Immunohistochemical staining for CD31 revealed that BTXA inhibited angiogenesis.
UNASSIGNED: HFUS and SWE displayed excellent performance in evaluating HS thickness and stiffness. SMI showed a good performance in reflecting microvascular signals in HS. These ultrasound techniques have great potential in assessing the therapeutic effect of BTXA in HS.
UNASSIGNED: Eight healthy New Zealand long-eared rabbits were utilized in the study. Four wounds, each measuring 1.0 cm in diameter, were created on both ears of each rabbit. Immediately after surgery, each of these wounds received an injection containing a distinct concentration of BTXA. On postoperative week 6, scar thickness, vascularity, and hardness were assessed based on high frequency ultrasound (HFUS), superb microvascular imaging (SMI), shear wave elastography (SWE), Masson staining, and immunohistochemical staining for CD31.
UNASSIGNED: All wounds healed well, and HSs formed after 6 weeks post-surgery. Scar thickness based on HFUS presented a significant decrease with increasing BTXA concentration (p < 0.05), aligning with the gross morphology. Simultaneously, scar stiffness, evaluated using SWE, showed a significant decrease in accordance with the variation of the collagen volume fraction, which refers to the ratio of the collagen positive area to the total area (p < 0.05). Although the vascularity index obtained by SMI did not exhibit a statistically significant change across different BTXA concentrations, this technique effectively illustrated the microvascular perfusion in HS. Immunohistochemical staining for CD31 revealed that BTXA inhibited angiogenesis.
UNASSIGNED: HFUS and SWE displayed excellent performance in evaluating HS thickness and stiffness. SMI showed a good performance in reflecting microvascular signals in HS. These ultrasound techniques have great potential in assessing the therapeutic effect of BTXA in HS.
摘要：
■验证超声在评估A型肉毒杆菌毒素（BTXA）治疗增生性瘢痕（HS）疗效中的可行性。
■研究中使用了八只健康的新西兰长耳兔。四个伤口,每个直径为1.0厘米，是在每只兔子的两只耳朵上产生的。手术后立即，这些伤口中的每一个都接受了含有不同浓度BTXA的注射。术后第6周，瘢痕厚度，血管，根据高频超声（HFUS）评估硬度，精湛的微血管成像（SMI），剪切波弹性成像（SWE），Masson染色,和CD31的免疫组织化学染色。
■所有伤口愈合良好，术后6周后形成HSs。基于HFUS的疤痕厚度随着BTXA浓度的增加而显著降低(p<0.05),与总体形态一致。同时,疤痕硬度,使用SWE评估，随着胶原蛋白体积分数的变化，指胶原阳性面积与总面积的比值(p<0.05)。尽管通过SMI获得的血管分布指数在不同的BTXA浓度下没有显示出统计学上的显着变化，该技术有效地说明了HS中的微血管灌注。CD31的免疫组织化学染色显示BTXA抑制血管生成。
■HFUS和SWE在评估HS厚度和刚度方面表现出出色的性能。SMI在反映HS中的微血管信号方面表现出良好的性能。这些超声技术在评估BTXA在HS中的治疗效果方面具有巨大的潜力。
■研究中使用了八只健康的新西兰长耳兔。四个伤口,每个直径为1.0厘米，是在每只兔子的两只耳朵上产生的。手术后立即，这些伤口中的每一个都接受了含有不同浓度BTXA的注射。术后第6周，瘢痕厚度，血管，根据高频超声（HFUS）评估硬度，精湛的微血管成像（SMI），剪切波弹性成像（SWE），Masson染色,和CD31的免疫组织化学染色。
■所有伤口愈合良好，术后6周后形成HSs。基于HFUS的疤痕厚度随着BTXA浓度的增加而显著降低(p<0.05),与总体形态一致。同时,疤痕硬度,使用SWE评估，随着胶原蛋白体积分数的变化，指胶原阳性面积与总面积的比值(p<0.05)。尽管通过SMI获得的血管分布指数在不同的BTXA浓度下没有显示出统计学上的显着变化，该技术有效地说明了HS中的微血管灌注。CD31的免疫组织化学染色显示BTXA抑制血管生成。
■HFUS和SWE在评估HS厚度和刚度方面表现出出色的性能。SMI在反映HS中的微血管信号方面表现出良好的性能。这些超声技术在评估BTXA在HS中的治疗效果方面具有巨大的潜力。
