PDF(Pubmed)
Abstract：
Background This study investigated the anticoagulant properties of sulfated chitosan derived from the internal bone of the spineless cuttlefish Sepiella inermis. Chitosan, a biopolymer, is used in various biomedical applications including anticoagulation. Sulfation of chitosan enhances its biological activity, making it a potential therapeutic agent. This study explored the efficacy of sulfated chitosan in preventing blood clot formation to provide a novel anticoagulant alternative. Objectives This study aimed to synthesize and characterize the anticoagulant properties of sulfated chitosan extracted from the internal bone of the spineless cuttlefish S. inermis using Fourier Transform Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FESEM), and X-Ray Diffraction (XRD) and evaluate the anticoagulant properties of sulfated chitosan extracted from the internal bone of spineless cuttlefish S. inermis. Materials and methods Chitin and chitosan were extracted from the cuttlebone of a specimen of S. inermis, and sulfated chitosan was synthesized by sulfation of chitosan. Sulfated chitosan was subsequently used to evaluate its anticoagulant properties using tests such as activated partial thromboplastin time (APTT) and prothrombin time (PT). Characteristic investigations were conducted, including FTIR, FESEM, and XRD analyses. Results The results of this study suggested the possibility of using S. inermis internal bone as an unconventional source of natural anticoagulant that can be combined with biomedical applications. Anticoagulant activity measured using APTT and PT showed that sulfated chitosan was a strong anticoagulant. Conclusion We examined the anticoagulant activity of S. inermis extract using thrombin and activated partial thromboplastin times. Our results demonstrated the heparin-like anticoagulant action of the extracted sulfated chitosan, suggesting that it may be a great alternative anticoagulant treatment.
摘要：
背景这项研究调查了来自无骨墨鱼Sepiellainermis内部骨骼的硫酸化壳聚糖的抗凝血特性。壳聚糖,一种生物聚合物，用于各种生物医学应用，包括抗凝。壳聚糖的硫酸化增强其生物活性,使其成为潜在的治疗剂。这项研究探索了硫酸化壳聚糖在防止血凝块形成中的功效，以提供一种新型的抗凝剂替代品。目的本研究旨在利用傅里叶变换红外光谱(FTIR)技术,合成并表征无骨墨鱼骨内硫酸化壳聚糖的抗凝血性能,场发射扫描电子显微镜(FESEM),和X射线衍射（XRD），并评估了从无骨墨鱼S.inermis内部骨提取的硫酸化壳聚糖的抗凝血性能。材料和方法从S.inermis标本的海绵体中提取甲壳素和壳聚糖，通过壳聚糖的硫酸化反应合成了硫酸化壳聚糖。硫酸化壳聚糖随后使用诸如活化部分凝血活酶时间(APTT)和凝血酶原时间(PT)的测试来评估其抗凝血性质。进行了特征调查，包括FTIR,FESEM，和XRD分析。结果这项研究的结果表明，使用S.inermis内部骨作为天然抗凝剂的非常规来源，可以与生物医学应用相结合。使用APTT和PT测量的抗凝血活性表明硫酸化壳聚糖是强抗凝剂。结论我们使用凝血酶和活化的部分凝血活酶时间检查了S.inermis提取物的抗凝血活性。我们的结果表明了提取的硫酸化壳聚糖的肝素样抗凝血作用，这表明它可能是一种很好的抗凝治疗方法。
