PDF(Pubmed)
Abstract：
BACKGROUND: Electronic cigarettes (e-cigarettes) are often advertised as a safer alternative to traditional smoking. However, recent data suggest they may not be as safe as previously believed. This study aims to evaluate the genotoxicity and mutagenicity of e-cigarette liquids.
METHODS: We randomly selected eight varieties of e-cigarette liquids from the local market in Jeddah, Saudi Arabia. We evaluated their genotoxicity using the Genotoxicity SOS-Chromo Test™ Kit. In this investigation, a rat liver S9 fraction was utilized to emulate liver metabolic function to measure any chemical substance\'s mutagenic potential. The SOS-Chromo Test was performed by recording the β-galactosidase and alkaline phosphatase activity with and without the metabolic activation enzyme (S-9).
RESULTS: All samples, except for the first two dilutions of sample 2, were nongenotoxic in the absence of the S9 activation enzyme, according to the genotoxicity analysis. However, when tested in the presence of the S9 enzyme, samples 2, 4, and 7 exhibited mutagenic activity at varying concentrations.
CONCLUSIONS: Contrary to common belief, e-cigarettes are not safe. The present investigation confirms the presence of both toxicants and carcinogens in some e-cigarette liquids. This exposure could increase users\' risk of various health complications.
METHODS: We randomly selected eight varieties of e-cigarette liquids from the local market in Jeddah, Saudi Arabia. We evaluated their genotoxicity using the Genotoxicity SOS-Chromo Test™ Kit. In this investigation, a rat liver S9 fraction was utilized to emulate liver metabolic function to measure any chemical substance\'s mutagenic potential. The SOS-Chromo Test was performed by recording the β-galactosidase and alkaline phosphatase activity with and without the metabolic activation enzyme (S-9).
RESULTS: All samples, except for the first two dilutions of sample 2, were nongenotoxic in the absence of the S9 activation enzyme, according to the genotoxicity analysis. However, when tested in the presence of the S9 enzyme, samples 2, 4, and 7 exhibited mutagenic activity at varying concentrations.
CONCLUSIONS: Contrary to common belief, e-cigarettes are not safe. The present investigation confirms the presence of both toxicants and carcinogens in some e-cigarette liquids. This exposure could increase users\' risk of various health complications.
摘要：
背景：电子烟（电子烟）通常被宣传为传统吸烟的更安全的替代品。然而,最近的数据表明，它们可能不像以前认为的那样安全。本研究旨在评估电子烟液体的遗传毒性和致突变性。
方法：我们从吉达当地市场随机选择了8种电子烟液，沙特阿拉伯。我们使用遗传毒性SOS-ChromoTest™试剂盒评估了它们的遗传毒性。在这次调查中,大鼠肝脏S9部分用于模拟肝脏代谢功能，以测量任何化学物质的诱变潜力。通过记录有和没有代谢活化酶(S-9)的β-半乳糖苷酶和碱性磷酸酶活性来进行SOS-染色体测试。
结果：所有样本，除了样品2的前两个稀释液，在没有S9激活酶的情况下是无遗传毒性的，根据基因毒性分析。然而,当在S9酶存在下测试时，样品2、4和7在不同浓度下表现出诱变活性。
结论：与普遍看法相反，电子烟不安全。本调查证实了某些电子烟液体中存在有毒物质和致癌物质。这种暴露可能会增加使用者患各种健康并发症的风险。
方法：我们从吉达当地市场随机选择了8种电子烟液，沙特阿拉伯。我们使用遗传毒性SOS-ChromoTest™试剂盒评估了它们的遗传毒性。在这次调查中,大鼠肝脏S9部分用于模拟肝脏代谢功能，以测量任何化学物质的诱变潜力。通过记录有和没有代谢活化酶(S-9)的β-半乳糖苷酶和碱性磷酸酶活性来进行SOS-染色体测试。
结果：所有样本，除了样品2的前两个稀释液，在没有S9激活酶的情况下是无遗传毒性的，根据基因毒性分析。然而,当在S9酶存在下测试时，样品2、4和7在不同浓度下表现出诱变活性。
结论：与普遍看法相反，电子烟不安全。本调查证实了某些电子烟液体中存在有毒物质和致癌物质。这种暴露可能会增加使用者患各种健康并发症的风险。
