PDF(Pubmed)
Abstract：
UNASSIGNED: Naringenin has shown great promise in the realm of cancer therapeutics, demonstrating excellent cytotoxic action toward cancer cells and the enhanced effects of radiation therapy in vitro. However, the medicinal value of naringenin is severely limited clinically by poor bioavailability. Thus, multiple drug-delivery strategies for overcoming this limitation have been developed, of which liposomes are considered the most suitable due to their amphiphilic, modifiable, and biocompatible characteristics. In this study, we investigated the role of naringenin and liposomal-delivered naringenin as adjuncts to radiotherapy in the MDA-MB-231 triple-negative breast cancer cell line in vitro.
UNASSIGNED: Liposomal-naringenin was synthesized by thin-film hydration and extrusion and was characterized by spectrophotometry, dynamic light scattering, and zeta potential. The effects of free-from naringenin and liposomal-naringenin were evaluated toward MDA-MB-231 cell viability when combined with varying doses of radiation. Additionally, cell growth patterns, morphology, and colony formation were evaluated.
UNASSIGNED: The analysis demonstrated IC50 values of 387.5 and 546.6 µg/ml for naringenin and liposomal-naringenin, respectively. Naringenin and liposomal-naringenin significantly lowered cell viability, proliferation, and colony formation dose-dependently, as compared to radiation in isolation.
UNASSIGNED: The findings presented herein concur with previous accounts of the radiosensitizing potential of naringenin and further highlight the considerable biomedical application of liposomal-naringenin within the realm of radiotherapy.
UNASSIGNED: Liposomal-naringenin was synthesized by thin-film hydration and extrusion and was characterized by spectrophotometry, dynamic light scattering, and zeta potential. The effects of free-from naringenin and liposomal-naringenin were evaluated toward MDA-MB-231 cell viability when combined with varying doses of radiation. Additionally, cell growth patterns, morphology, and colony formation were evaluated.
UNASSIGNED: The analysis demonstrated IC50 values of 387.5 and 546.6 µg/ml for naringenin and liposomal-naringenin, respectively. Naringenin and liposomal-naringenin significantly lowered cell viability, proliferation, and colony formation dose-dependently, as compared to radiation in isolation.
UNASSIGNED: The findings presented herein concur with previous accounts of the radiosensitizing potential of naringenin and further highlight the considerable biomedical application of liposomal-naringenin within the realm of radiotherapy.
摘要：
■柚皮素在癌症治疗领域显示出巨大的希望，对癌细胞表现出优异的细胞毒性作用，并在体外增强放射治疗的效果。然而,柚皮素的药用价值在临床上受到生物利用度差的严重限制。因此，已经开发了克服这一限制的多种药物递送策略，其中脂质体由于其两亲性而被认为是最合适的，可修改，和生物相容性特征。在这项研究中,我们在体外研究了柚皮素和脂质体递送的柚皮素在MDA-MB-231三阴性乳腺癌细胞系中作为放疗辅助物的作用.
■脂质体-柚皮素是通过薄膜水合和挤出合成的，并通过分光光度法进行了表征，动态光散射,和zeta电位。当与不同剂量的辐射组合时，评估了游离柚皮素和脂质体-柚皮素对MDA-MB-231细胞活力的影响。此外,细胞生长模式，形态学,和集落形成进行了评价。
■分析显示柚皮素和脂质体-柚皮素的IC50值为387.5和546.6µg/ml，分别。柚皮素和脂质体柚皮素显著降低细胞活力，扩散,和集落形成剂量依赖性，与孤立的辐射相比。
本文呈现的发现与先前关于柚皮素的放射增敏潜力的描述一致,并且进一步突出了脂质体-柚皮素在放射治疗领域中的相当大的生物医学应用。
■脂质体-柚皮素是通过薄膜水合和挤出合成的，并通过分光光度法进行了表征，动态光散射,和zeta电位。当与不同剂量的辐射组合时，评估了游离柚皮素和脂质体-柚皮素对MDA-MB-231细胞活力的影响。此外,细胞生长模式，形态学,和集落形成进行了评价。
■分析显示柚皮素和脂质体-柚皮素的IC50值为387.5和546.6µg/ml，分别。柚皮素和脂质体柚皮素显著降低细胞活力，扩散,和集落形成剂量依赖性，与孤立的辐射相比。
本文呈现的发现与先前关于柚皮素的放射增敏潜力的描述一致,并且进一步突出了脂质体-柚皮素在放射治疗领域中的相当大的生物医学应用。
