PDF(Pubmed)
Abstract：
Brain damage triggers diverse cellular and molecular events, with astrocytes playing a crucial role in activating local neuroprotective and reparative signaling within damaged neuronal circuits. Here, we investigated reactive astrocytes using a multidimensional approach to categorize their responses into different subtypes based on morphology. This approach utilized the StarTrack lineage tracer, single-cell imaging reconstruction and multivariate data analysis. Our findings identified three profiles of reactive astrocyte responses, categorized by their effects on cell size- and shape- related morphological parameters: \"moderate\", \"strong,\" and \"very strong\". We also examined the heterogeneity of astrocyte reactivity, focusing on spatial and clonal distribution. Our research revealed a notable enrichment of protoplasmic and fibrous astrocytes within the \"strong\" and \"very strong\" response subtypes. Overall, our study contributes to a better understanding of astrocyte heterogeneity in response to an injury. By characterizing the diverse reactive responses among astrocyte subpopulations, we provide insights that could guide future research aimed at identifying novel therapeutic targets to mitigate brain damage and promote neural repair.
摘要：
脑损伤引发不同的细胞和分子事件，星形胶质细胞在激活受损神经元回路内的局部神经保护和修复信号中起着至关重要的作用。这里,我们使用多维方法研究了反应性星形胶质细胞,根据形态学将其反应分为不同亚型.这种方法利用了StarTrack谱系示踪剂，单细胞成像重建和多变量数据分析。我们的发现确定了三种反应性星形胶质细胞反应的概况，根据它们对细胞大小和形状相关形态参数的影响进行分类：“中度”，\"坚强,\"和\"非常强大\"。我们还检查了星形胶质细胞反应性的异质性，侧重于空间和克隆分布。我们的研究表明，在“强”和“非常强”反应亚型中，原生质和纤维星形胶质细胞的显着富集。总的来说,我们的研究有助于更好地理解星形胶质细胞对损伤的反应异质性.通过表征星形胶质细胞亚群之间的不同反应性反应，我们提供的见解可以指导未来的研究，旨在确定新的治疗靶点，以减轻脑损伤和促进神经修复。
