PDF(Pubmed)
Abstract：
Inferring gene regulatory networks from single-cell RNA-sequencing trajectories has been an active area of research yet methods are still needed to identify regulators governing cell transitions. We developed DREAMIT (Dynamic Regulation of Expression Across Modules in Inferred Trajectories) to annotate transcription-factor activity along single-cell trajectory branches, using ensembles of relations to target genes. Using a benchmark representing several different tissues, as well as external validation with ATAC-Seq and Perturb-Seq data on hematopoietic cells, the method was found to have higher tissue-specific sensitivity and specificity over competing approaches.
摘要：
从单细胞RNA测序轨迹推断基因调控网络一直是一个活跃的研究领域，但仍需要方法来识别调控细胞转换的调控因子。我们开发了DREAMIT（在推断轨迹中跨模块表达的动态调节）来注释沿单细胞轨迹分支的转录因子活性，使用关系集合到目标基因。使用代表几种不同组织的基准，以及对造血细胞的ATAC-Seq和Perturb-Seq数据的外部验证，发现该方法比竞争方法具有更高的组织特异性敏感性和特异性.
