Abstract：
Modifying cells with polymers on the surface can enable them to gain or enhance function with various applications, wherein the atom transfer radical polymerization (ATRP) has garnered significant potential due to its biocompatibility. However, specifically initiating ATRP from the cell surface for in-situ modification remains challenging. This study established a bacterial surface-initiated ATRP method and further applied it for enhanced Cr(VI) removal. The cell surface specificity was facilely achieved by cell surface labelling with azide substrates, following alkynyl ATRP initiator specifically anchoring with azide-alkyne click chemistry. Then, the ATRP polymerization was initiated from the cell surface, and different polymers were successfully applied to in-situ modification. Further analysis revealed that the modification of Shewanella oneidensis with poly (4-vinyl pyridine) and sodium polymethacrylate improved the heavy metal tolerance and enhanced the Cr(VI) removal rate of 2.6 times from 0.088 h-1 to 0.314 h-1. This work provided a novel idea for bacterial surface modification and would extend the application of ATRP in bioremediation.
摘要：
用表面上的聚合物修饰细胞可以使它们在各种应用中获得或增强功能，其中原子转移自由基聚合(ATRP)由于其生物相容性而获得了显著的潜力。然而,从细胞表面特异性启动ATRP进行原位修饰仍然具有挑战性。这项研究建立了细菌表面引发的ATRP方法，并将其进一步应用于增强的Cr（VI）去除。通过用叠氮化物底物标记细胞表面很容易实现细胞表面特异性，在用叠氮化物-炔点击化学特异性锚定的炔基ATRP引发剂之后。然后，ATRP聚合从细胞表面开始，并将不同的聚合物成功地应用于原位改性。进一步的分析表明，用聚（4-乙烯基吡啶）和聚甲基丙烯酸钠改性希瓦氏菌可以提高重金属耐受性，并将Cr（VI）的去除率从0.088h-1提高到0.314h-1，提高了2.6倍。这项工作为细菌表面改性提供了新的思路，并将扩展ATRP在生物修复中的应用。
