Abstract：
Excessive use of antibiotics will enter the water environment and soil through the biological chain, and then transfer to the human body through food, resulting in drug resistance, kidney toxicity and other health problems, so it is urgent to develop highly sensitive detection methods of antibiotics. Here, we designed a dual-mode sensor platform based on closed bipolar electrode (cBPE) electroluminescence (ECL) and mobile phone imaging to detect kanamycin in seawater. The prepared CN-NV-550 displayed extremely intense ECL signal, allowing for convenient mobile phone imaging. The cBPE was combined with DNA cycle amplification technology to prevent the mutual interference between target and the luminescent material, and realized the amplification of signal. In the presence of target Kana, Co3O4 was introduced to the cBPE anode by DNA cycle amplification product, and accelerated the oxidation rate of uric acid (UA). Thus, the electroluminescence response of CN-NV-550 on cBPE cathode was much improved due to the charge balance of the cBPE, achieving both ECL detection and mobile phone imaging assay of Kana, which much improved the accuracy and efficiency of assay. The limit of detection (LOD) in this work is 0.23 pM, and LOD for mobile phone imaging is 0.39 pM. This study integrate ECL imaging visualization of CN-NV-550 and high electrocatalytic activity of Co3O4 into cBPE-ECL detection, providing a new perspective for antibiotic analysis, and has great potential for practical applications, especially in Marine environmental pollution monitoring.
摘要：
抗生素的过度使用会通过生物链进入水环境和土壤，然后通过食物转移到人体，导致耐药性，肾毒性和其他健康问题，因此，迫切需要开发高灵敏度的抗生素检测方法。这里,我们设计了基于封闭双极电极（cBPE）电致发光（ECL）和手机成像的双模式传感器平台，以检测海水中的卡那霉素。制备的CN-NV-550显示出极其强烈的ECL信号，允许方便的手机成像。将cBPE与DNA循环扩增技术相结合，以防止靶标与发光材料之间的相互干扰，实现了信号的放大。在目标假名存在的情况下，通过DNA循环扩增产物将Co3O4引入cBPE阳极，并加快了尿酸(UA)的氧化速率。因此，由于cBPE的电荷平衡，CN-NV-550在cBPE阴极上的电致发光响应得到了很大的改善，实现了假名的ECL检测和手机成像检测，大大提高了检测的准确性和效率。这项工作的检测限（LOD）为0.23pM，移动电话成像的LOD为0.39pM。本研究将CN-NV-550的ECL成像可视化和Co3O4的高电催化活性整合到cBPE-ECL检测中，为抗生素分析提供了新的视角，并具有巨大的实际应用潜力，特别是海洋环境污染监测。
