Abstract：
This study presents a thrombin-loaded cationized chitosan (TCCS) sponge with highly effective hemostatic and antibacterial activity. The TCCS sponge, prepared using a multistep method, features a porous structure, favorable mechanical properties, excellent water absorption ability, and shape recovery triggered by water or blood. The TCCS sponge exhibited strong antibacterial activity against Methicillin-resistant Staphylococcus aureus and Escherichia coli. Additionally, it demonstrated enhanced procoagulant and hemostatic efficacy in rat tail amputation and rat liver perforation wound models compared to commercial hemostats. Furthermore, the sponge exhibited favorable biocompatibility and biosafety. These findings suggest that the TCCS sponge has substantial potential for practical applications in managing severe hemorrhages and bacterial infections.
摘要：
这项研究提出了一种负载凝血酶的阳离子化壳聚糖（TCCS）海绵，具有高效的止血和抗菌活性。TCCS海绵，使用多步法制备，具有多孔结构，良好的机械性能，优异的吸水能力，由水或血液引发的形状恢复。TCCS海绵对耐甲氧西林金黄色葡萄球菌和大肠杆菌具有较强的抗菌活性。此外,与商用止血剂相比，它在大鼠尾部截肢和大鼠肝穿孔伤口模型中显示出增强的促凝血和止血功效。此外，海绵表现出良好的生物相容性和生物安全性。这些发现表明，TCCS海绵在管理严重出血和细菌感染方面具有实际应用的巨大潜力。
