Abstract：
It aims to prepare the chitosan (CS) and polyethylene oxide (PEO) hydrogel membranes with different CS/PEO blend ratios (100:0, 95:5, 90:10, 80:20 and 70:30) via solvent casting. The physicochemical properties of these membranes were investigated using various characterization techniques: Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive X-ray (EDX), contact angle, and tensile testing. The interaction of PEO and chitosan was investigated by DSC in terms of freezing bound, freezing free, and non-freezing PEO fraction. The cross-sectional surface morphology of membranes displayed a smoother surface with increasing PEO content up to 20 %, beyond which nonhomogeneity on the surface was visible. The antifouling behavior of membranes was investigated by bacterial adherence study, which showed an enhanced antifouling nature of membranes with the increase in the PEO content. The peeling strength of the membranes was measured using a 90° angle peeling test, and it was found that 20 % and more PEO content promotes easy removal from the gelatin slab. In addition to this, live/ dead assay of the CS was performed to visualize the presence of live and dead bacteria on the surface. The CS/PEO blend with 20 % PEO content has properties makes it suitable for use as a protective layer on wound dressings to prevent bacterial growth. It\'s use in wound dressings has the potential to reduce the pain during the time of dressing removal and improve patient outcomes. The present investigation leads to the development of a CS hydrogel matrix which exhibits very interesting interaction with the PEO moiety along with its innovative feature of antifouling and antimicrobial nature.
摘要：
目的通过溶剂流延法制备具有不同CS/PEO混合比（100：0、95：5、90：10、80：20和70：30）的壳聚糖（CS）和聚环氧乙烷（PEO）水凝胶膜。使用各种表征技术研究了这些膜的物理化学性质：傅里叶变换红外光谱（FTIR），X射线衍射(XRD)差示扫描量热法(DSC),扫描电子显微镜（SEM），原子力显微镜（AFM），能量色散X射线（EDX），接触角,和拉伸测试。通过DSC研究了PEO和壳聚糖在冻结结合方面的相互作用，无冻结,和非冻结PEO馏分。随着PEO含量的增加，膜的横截面表面形态显示出更光滑的表面，高达20%，除此之外，表面上的不均匀性是可见的。通过细菌粘附研究研究了膜的抗污染行为，随着PEO含量的增加，膜的抗污性增强。使用90°角剥离试验测量膜的剥离强度。并且发现20%和更多的PEO含量促进从明胶板的容易去除。除此之外,进行CS的活/死测定以显现表面上活的和死的细菌的存在。具有20%PEO含量的CS/PEO共混物具有使其适合用作伤口敷料上的保护层以防止细菌生长的性质。它在伤口敷料中的使用有可能减轻敷料移除期间的疼痛并改善患者的预后。本研究导致了CS水凝胶基质的开发，该基质与PEO部分表现出非常有趣的相互作用以及其防污和抗微生物性质的创新特征。
