Abstract：
Cleavage Under Targets and Tagmentation (CUT&Tag) is a recent methodology used for robust epigenomic profiling that, unlike conventional chromatin immunoprecipitation (ChIP-Seq), requires only a limited amount of cells as starting material. RNA sequencing (RNA-Seq) reveals the presence and quantity of RNA in a biological sample, describing the continuously changing cellular transcriptome. The integrated analysis of transcriptional activity, histone modifications, and chromatin accessibility via CUT&Tag is still in its infancy compared to the well-established ChIP-Seq. This chapter describes a robust bioinformatics methodology and workflow to perform an integrative CUT&Tag/RNA-Seq analysis.
摘要：
目标和标签下的切割(CUT&Tag)是一种用于强大的表观基因组分析的最新方法，与传统的染色质免疫沉淀（ChIP-Seq）不同，只需要有限量的细胞作为起始材料。RNA测序（RNA-Seq）揭示了生物样品中RNA的存在和数量，描述不断变化的细胞转录组。转录活性的综合分析，组蛋白修饰，与完善的ChIP-Seq相比，通过CUT和Tag的染色质可及性仍处于起步阶段。本章介绍了一种强大的生物信息学方法和工作流程，以执行综合CUT&Tag/RNA-Seq分析。
