Abstract：
UNASSIGNED: Direct evidence for the relationship between a large prostate (≥80 ml) and androgen receptor/PSA signal remains lacking in benign prostatic hyperplasia (BPH). Our aim is to identify whether the cause of a large prostate is related to progesterone receptor (PGR) androgen receptor (AR), oestrogen receptor α, β (ERα,β) and prostate-specific antigen (PSA).
UNASSIGNED: Surgical specimens of BPH in plasmakinetic resection of the prostate (PKRP) with three groups of different prostate-sizes with mean volumes of 25.97 ml, 63.80 ml, and 122.37 ml were collected for immunohistochemical analysis of the tissue microarray with PGR, AR, PSA and ERs. Rats were castrated and treated with testosterone replacement to explore androgen and PGR, AR and ERs expression levels in the prostate. Quantitative real-time reverse transcription polymerase chain reaction (Rt-PCR) for mRNA detection of above genes was conducted.
UNASSIGNED: Immunoblotting, Rt-PCR and immunohistochemistry assays showed that PGR, PSA, AR, ERα expression levels were positively correlated with prostate size and that ERβ expression levels were negatively correlated with prostate volume. Animal experiments have shown that prostate volume is decreased in castrated rats with decreased PGR, AR, ERα and increased ERβ expression levels.
UNASSIGNED: PGR, AR, ERs signals can be regarded as important factors for large-sized prostates in BPH patients (≥100 ml).
UNASSIGNED: Surgical specimens of BPH in plasmakinetic resection of the prostate (PKRP) with three groups of different prostate-sizes with mean volumes of 25.97 ml, 63.80 ml, and 122.37 ml were collected for immunohistochemical analysis of the tissue microarray with PGR, AR, PSA and ERs. Rats were castrated and treated with testosterone replacement to explore androgen and PGR, AR and ERs expression levels in the prostate. Quantitative real-time reverse transcription polymerase chain reaction (Rt-PCR) for mRNA detection of above genes was conducted.
UNASSIGNED: Immunoblotting, Rt-PCR and immunohistochemistry assays showed that PGR, PSA, AR, ERα expression levels were positively correlated with prostate size and that ERβ expression levels were negatively correlated with prostate volume. Animal experiments have shown that prostate volume is decreased in castrated rats with decreased PGR, AR, ERα and increased ERβ expression levels.
UNASSIGNED: PGR, AR, ERs signals can be regarded as important factors for large-sized prostates in BPH patients (≥100 ml).
摘要：
■在良性前列腺增生（BPH）中缺乏大前列腺（≥80ml）与雄激素受体/PSA信号之间关系的直接证据。我们的目的是确定大前列腺的原因是否与孕激素受体（PGR）雄激素受体（AR）有关，雌激素受体α，β（ERα，β）和前列腺特异性抗原（PSA）。
■前列腺等离子切除术（PKRP）中BPH的手术标本，三组不同的前列腺大小，平均体积为25.97ml，63.80ml,收集122.37ml用于PGR组织微阵列的免疫组织化学分析，AR,PSA和ER。去势大鼠，用睾酮替代治疗，以探索雄激素和PGR，前列腺中AR和ERs的表达水平。进行定量实时逆转录聚合酶链反应(Rt-PCR)以检测上述基因的mRNA。
■免疫印迹，Rt-PCR和免疫组织化学检测显示PGR,PSA,AR,ERα表达水平与前列腺大小呈正相关，ERβ表达水平与前列腺体积呈负相关。动物实验表明，PGR降低的去势大鼠前列腺体积减小，AR,ERα和ERβ表达水平增加。
■PGR，AR,ERs信号可被视为BPH患者（≥100ml）中大型前列腺的重要因素。
■前列腺等离子切除术（PKRP）中BPH的手术标本，三组不同的前列腺大小，平均体积为25.97ml，63.80ml,收集122.37ml用于PGR组织微阵列的免疫组织化学分析，AR,PSA和ER。去势大鼠，用睾酮替代治疗，以探索雄激素和PGR，前列腺中AR和ERs的表达水平。进行定量实时逆转录聚合酶链反应(Rt-PCR)以检测上述基因的mRNA。
■免疫印迹，Rt-PCR和免疫组织化学检测显示PGR,PSA,AR,ERα表达水平与前列腺大小呈正相关，ERβ表达水平与前列腺体积呈负相关。动物实验表明，PGR降低的去势大鼠前列腺体积减小，AR,ERα和ERβ表达水平增加。
■PGR，AR,ERs信号可被视为BPH患者（≥100ml）中大型前列腺的重要因素。
