PDF(Pubmed)
Abstract：
Various metabolic diseases caused by a high-fat diet (HFD) are closely related to gut microbiota dysbiosis and epithelial barrier dysfunction. Polycan, a type of β-glucan, is effective in treating anti-obesity and metabolic diseases caused by HFD. However, the effect of Polycan on dysbiosis and epithelial barrier damage is still unknown. In this study, the effects of Polycan on dysbiosis and intestinal barrier damage were investigated using HFD-induced obese model mice. C57BL/6 mice were fed a HFD for 12 weeks and treated with two different doses of Polycan (250 and 500 mg/kg) orally administered during weeks 9 to 12. Polycan supplementation increased the expression of tight junction genes (zonula occludens-1, occludin, and claudin-3) and short-chain fatty acid (SCFA) content while reducing toxic substances (phenol, p-cresol, and skatole). Most significantly, Polycan enriched SCFA-producing bacteria (i.e., Phocaeicola, Bacteroides, Faecalibaculum, Oscillibacter, Lachnospiraceae, and Muribaculaceae), and decreased the Firmicutes/Bacteroidetes ratio and toxic substances-producing bacteria (i.e., Olsenella, Clostridium XVIII, and Schaedlerella). Furthermore, microbial functional capacity prediction of the gut microbiota revealed that Polycan enriched many SCFA-related KEGG enzymes while toxic substance-related KEGG enzymes were depleted. These findings indicated that Polycan has the potential to alleviate HFD-induced intestinal barrier damage by modulating the function and composition of the gut microbiota.
摘要：
高脂饮食(HFD)引起的各种代谢性疾病与肠道菌群失调和上皮屏障功能障碍密切相关。Polycan,一种β-葡聚糖,有效治疗由HFD引起的抗肥胖和代谢性疾病。然而,Polycan对菌群失调和上皮屏障损伤的影响尚不清楚。在这项研究中,使用HFD诱导的肥胖模型小鼠研究了Polycan对菌群失调和肠屏障损伤的影响。给C57BL/6小鼠喂食HFD12周,并在第9至12周期间口服施用两种不同剂量的Polycan(250和500mg/kg)。Polycan补充增加了紧密连接基因的表达（zonulaoccludens-1，occludin，和claudin-3）和短链脂肪酸（SCFA）含量，同时减少有毒物质（苯酚，对甲酚,和skatole）。最重要的是，富含SCFA的产生菌(即，Phocaeicola,拟杆菌,Faecalibaculum,镰刀菌,落叶松科,和Muribaculaceae），并降低了Firmicutes/拟杆菌的比例和产生有毒物质的细菌(即，Olsenella,ClostridiumXVIII,和Schaedlella）。此外，肠道微生物群的微生物功能能力预测显示，Polycan富集了许多与SCFA相关的KEGG酶，而与毒性物质相关的KEGG酶被耗尽。这些发现表明Polycan具有通过调节肠道微生物群的功能和组成来减轻HFD诱导的肠屏障损伤的潜力。
