UNASSIGNED: The expression of Circ_BBS9 was examined in tissues and cell lines of LUAD through the utilization of microarray profiling, quantitative real-time polymerase chain reaction (qRT-PCR), and western blot analysis. In this study, we assessed the impact of circ_BBS9 on the proliferation of LUAD cells, as well as its influence on ferroptosis and tumor formation. To analyze these effects, we employed CCK-8 assays and ferroptosis assays. The identification of proteins that interact with Circ_BBS9 was achieved through the utilization of RNA pull-down and mass spectrometry techniques. A putative regulatory network comprising circ_BBS9, miR-7150, and IFIT3 was established using bioinformatics study. The investigation also encompassed the examination of the correlation between the expression of IFIT3 and the invasion of immune cells.
UNASSIGNED: Circ_BBS9 was significantly downregulated in LUAD tissues and cell lines. Low circ_BBS9 expression correlated with poor prognosis. Functional experiments showed that circ_BBS9 overexpression inhibited LUAD cell proliferation and promoted ferroptosis in vitro and suppressed tumor growth in vivo. Mechanistically, circ_BBS9 was found to directly interact with IFIT3 and regulate its expression by acting as a sponge for miR-7150. Additionally, IFIT3 expression correlated positively with immune infiltration in LUAD.
UNASSIGNED: Circ_BBS9 has been identified as a tumor suppressor in lung adenocarcinoma (LUAD) and holds promise as a diagnostic biomarker. The potential mechanism of action involves the modulation of ferroptosis and the immunological microenvironment through direct interaction with IFIT3 and competitive binding to miR-7150. The aforementioned findings offer new perspectives on the pathophysiology of LUAD and highlight circ_BBS9 as a potentially valuable target for therapeutic interventions.
■通过利用微阵列分析在LUAD的组织和细胞系中检查Circ_BBS9的表达,定量实时聚合酶链反应(qRT-PCR),和蛋白质印迹分析。在这项研究中,我们评估了circ_BBS9对LUAD细胞增殖的影响,以及它对铁死亡和肿瘤形成的影响。为了分析这些影响,我们采用CCK-8测定和铁凋亡测定。通过利用RNA下拉和质谱技术实现与Circ_BBS9相互作用的蛋白质的鉴定。使用生物信息学研究建立了包含circ_BBS9、miR-7150和IFIT3的推定调控网络。该研究还包括检查IFIT3的表达与免疫细胞侵袭之间的相关性。
■Circ_BBS9在LUAD组织和细胞系中显著下调。低circ_BBS9表达与不良预后相关。功能实验表明,circ_BBS9过表达在体外抑制LUAD细胞增殖,促进铁凋亡,在体内抑制肿瘤生长。机械上,发现circ_BBS9直接与IFIT3相互作用,并通过充当miR-7150的海绵来调节其表达。此外,LUAD中IFIT3的表达与免疫浸润呈正相关。
■Circ_BBS9已被确定为肺腺癌(LUAD)的肿瘤抑制因子,并有望作为诊断生物标志物。潜在的作用机制涉及通过与IFIT3的直接相互作用和与miR-7150的竞争性结合来调节铁凋亡和免疫微环境。上述发现为LUAD的病理生理学提供了新的观点,并强调了circ_BBS9作为治疗干预的潜在有价值的目标。