Abstract：
Dermatophytosis is a prevalent fungal infection and public health burden, majorly caused by the attack of zoophilic fungi genera of Trichophyton and Microsporum. Among them, T. mentagrophytes and M. canis are the dominating pathogens that cause dermatophytosis in humans. Though anti-fungal treatments are available, the widespread drug resistance and minimal efficacy of conventional therapies cause recurring infections. In addition, prolonged anti-fungal medications induce several systemic side effects, including hepatotoxicity and leucopenia. The anti-dermatophytic formulation of biocompatible essential oil components (EOCs) is attractive due to their highly potent anti-dermatophytic action. Herein, two EOCs, Eugenol (EU) and Isoeugenol (IU), incorporated emulsion hydrogel (EOCs-EHG) synthesized from hydroxypropylmethyl cellulose and poly(ethylene glycol) methyl ether methacrylate. The cytocompatibility of the hydrogels is confirmed by treating them with fibroblast and keratinocyte cell lines. The EOCs-EHG demonstrated pH and temperature-responsive sustained release of entrapped EOCs and inhibited fungal spore germination. T. mentagrophytes and M. canis biofilms are eradicated at a minimal inhibitory concentration of 2 µg mL-1 each of EU and IU. The in vivo anti-dermatophytic activity of EOCs-EHG is confirmed in dermatophyte-infected Wistar albino rat models. The topical application of EOCs-EHG demonstrated complete infection eradication and facilitated skin regeneration, emphasizing the therapeutic potential of EOCs-EHG against dermatophytosis.
摘要：
皮肤癣菌病是一种常见的真菌感染和公共卫生负担，主要由毛癣菌和小孢子菌的嗜人真菌属的攻击引起。其中,植绿杆菌和犬分枝杆菌是导致人类皮肤癣菌病的主要病原体。虽然抗真菌治疗是可用的，广泛的耐药性和常规疗法的最低疗效导致反复感染。此外,长期服用抗真菌药物会引起一些全身副作用，包括肝毒性和白细胞减少症。生物相容性精油成分（EOC）的抗皮肤癣菌制剂由于其高效的抗皮肤癣菌作用而具有吸引力。在这里，两个EOC,丁香酚（EU）和异丁香酚（IU），由羟丙基甲基纤维素和聚（乙二醇）甲基醚甲基丙烯酸酯合成的掺入乳液水凝胶（EOCs-EHG）。水凝胶的细胞相容性通过用成纤维细胞和角质形成细胞系处理来确认。EOCs-EHG显示了对pH和温度敏感的包埋EOCs的持续释放，并抑制了真菌孢子的萌发。在EU和IU各自的2µgmL-1的最小抑制浓度下根除了T.在皮肤癣菌感染的Wistar白化病大鼠模型中证实了EOCs-EHG的体内抗皮肤癣菌活性。EOCs-EHG的局部应用显示完全根除感染并促进皮肤再生，强调EOCs-EHG对皮肤癣菌病的治疗潜力。
