PDF(Pubmed)
Abstract：
OBJECTIVE: The purpose of this study is to use two-sample Mendelian randomization (MR) to investigate the causal relationship between skin microbiota, especially Propionibacterium acnes, and intervertebral disc degeneration (IVDD), low back pain (LBP) and sciatica.
METHODS: We conducted a two-sample MR using the aggregated data from the whole genome-wide association studies (GWAS). 150 skin microbiota were derived from the GWAS catalog and IVDD, LBP and sciatica were obtained from the IEU Open GWAS project. Inverse-variance weighted (IVW) was the primary research method, with MR-Egger and Weighted median as supplementary methods. Perform sensitivity analysis and reverse MR analysis on all MR results and use multivariate MR to adjust for confounding factors.
RESULTS: MR revealed five skin microbiota associated with IVDD, four associated with LBP, and two with sciatica. Specifically, P.acnes in sebaceous skin environments were associated with reduced risk of IVDD; IVDD was found to increase the abundance of P.acnes in moist skin. Furthermore, ASV010 [Staphylococcus (unc.)] from dry skin was a risk factor for LBP and sciatica; ASV045 [Acinetobacter (unc.)] from dry skin and Genus Rothia from dry skin exhibited potential protective effects against LBP; ASV065 [Finegoldia (unc.)] from dry skin was a protective factor for IVDD and LBP. ASV054 [Enhydrobacter (unc.)] from moist skin, Genus Bacteroides from dry skin and Genus Kocuria from dry skin were identified as being associated with an increased risk of IVDD. Genus Streptococcus from moist skin was considered to be associated with an increased risk of sciatica.
CONCLUSIONS: This study identified a potential causal relationship between skin microbiota and IVDD, LBP, and sciatica. No evidence suggests skin-derived P.acnes is a risk factor for IVDD, LBP and sciatica. At the same time, IVDD can potentially cause an increase in P.acnes abundance, which supports the contamination theory.
METHODS: We conducted a two-sample MR using the aggregated data from the whole genome-wide association studies (GWAS). 150 skin microbiota were derived from the GWAS catalog and IVDD, LBP and sciatica were obtained from the IEU Open GWAS project. Inverse-variance weighted (IVW) was the primary research method, with MR-Egger and Weighted median as supplementary methods. Perform sensitivity analysis and reverse MR analysis on all MR results and use multivariate MR to adjust for confounding factors.
RESULTS: MR revealed five skin microbiota associated with IVDD, four associated with LBP, and two with sciatica. Specifically, P.acnes in sebaceous skin environments were associated with reduced risk of IVDD; IVDD was found to increase the abundance of P.acnes in moist skin. Furthermore, ASV010 [Staphylococcus (unc.)] from dry skin was a risk factor for LBP and sciatica; ASV045 [Acinetobacter (unc.)] from dry skin and Genus Rothia from dry skin exhibited potential protective effects against LBP; ASV065 [Finegoldia (unc.)] from dry skin was a protective factor for IVDD and LBP. ASV054 [Enhydrobacter (unc.)] from moist skin, Genus Bacteroides from dry skin and Genus Kocuria from dry skin were identified as being associated with an increased risk of IVDD. Genus Streptococcus from moist skin was considered to be associated with an increased risk of sciatica.
CONCLUSIONS: This study identified a potential causal relationship between skin microbiota and IVDD, LBP, and sciatica. No evidence suggests skin-derived P.acnes is a risk factor for IVDD, LBP and sciatica. At the same time, IVDD can potentially cause an increase in P.acnes abundance, which supports the contamination theory.
摘要：
目的：本研究的目的是使用两个样本孟德尔随机化（MR）来调查皮肤微生物群之间的因果关系，尤其是痤疮丙酸杆菌,和椎间盘退变（IVDD），腰痛（LBP）和坐骨神经痛。
方法：我们使用来自全基因组关联研究（GWAS）的汇总数据进行了双样本MR。150个皮肤微生物群来自GWAS目录和IVDD，从IEUOpenGWAS项目获得LBP和坐骨神经痛。逆方差加权(IVW)是主要的研究方法，用MR-Egger和加权中位数作为补充方法。对所有MR结果进行敏感性分析和反向MR分析，并使用多变量MR调整混杂因素。
结果：MR显示与IVDD相关的五种皮肤微生物群，四个与LBP有关，还有两个有坐骨神经痛.具体来说,皮脂腺皮肤环境中的痤疮丙酸杆菌与IVDD的风险降低相关;发现IVDD增加湿润皮肤中痤疮丙酸杆菌的丰度。此外，ASV010[葡萄球菌属(UNC.）]皮肤干燥是LBP和坐骨神经痛的危险因素；ASV045[不动杆菌（unc。）]来自干性皮肤和来自干性皮肤的Rothia属对LBP表现出潜在的保护作用；ASV065[Finegoldia（unc。)]皮肤干燥是IVDD和LBP的保护因素。ASV054[Enhydrobacter(unc.)]来自潮湿的皮肤，来自干性皮肤的拟杆菌属和来自干性皮肤的Kocuria属被鉴定为与IVDD的风险增加相关。来自潮湿皮肤的链球菌被认为与坐骨神经痛的风险增加有关。
结论：这项研究确定了皮肤微生物群与IVDD之间的潜在因果关系，LBP,和坐骨神经痛.没有证据表明皮肤源性痤疮丙酸杆菌是IVDD的危险因素，LBP和坐骨神经痛。同时,IVDD可能会导致痤疮丙酸杆菌丰度增加，这支持了污染理论。
方法：我们使用来自全基因组关联研究（GWAS）的汇总数据进行了双样本MR。150个皮肤微生物群来自GWAS目录和IVDD，从IEUOpenGWAS项目获得LBP和坐骨神经痛。逆方差加权(IVW)是主要的研究方法，用MR-Egger和加权中位数作为补充方法。对所有MR结果进行敏感性分析和反向MR分析，并使用多变量MR调整混杂因素。
结果：MR显示与IVDD相关的五种皮肤微生物群，四个与LBP有关，还有两个有坐骨神经痛.具体来说,皮脂腺皮肤环境中的痤疮丙酸杆菌与IVDD的风险降低相关;发现IVDD增加湿润皮肤中痤疮丙酸杆菌的丰度。此外，ASV010[葡萄球菌属(UNC.）]皮肤干燥是LBP和坐骨神经痛的危险因素；ASV045[不动杆菌（unc。）]来自干性皮肤和来自干性皮肤的Rothia属对LBP表现出潜在的保护作用；ASV065[Finegoldia（unc。)]皮肤干燥是IVDD和LBP的保护因素。ASV054[Enhydrobacter(unc.)]来自潮湿的皮肤，来自干性皮肤的拟杆菌属和来自干性皮肤的Kocuria属被鉴定为与IVDD的风险增加相关。来自潮湿皮肤的链球菌被认为与坐骨神经痛的风险增加有关。
结论：这项研究确定了皮肤微生物群与IVDD之间的潜在因果关系，LBP,和坐骨神经痛.没有证据表明皮肤源性痤疮丙酸杆菌是IVDD的危险因素，LBP和坐骨神经痛。同时,IVDD可能会导致痤疮丙酸杆菌丰度增加，这支持了污染理论。
