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Abstract：
Nanotechnology is a rapidly evolving field and has been extensively studied in biological applications. An understanding of the factors that influence nanoparticle diffusion in biofluids can aid in the development of diverse technologies. The development of real-time, label-free tracking technologies would allow the expansion of current knowledge of the diffusion and activity of nanoparticles. Fluorescence-based microscopy is one of the most widespread tools to monitor and track nanoparticle dynamics; however, the influence of fluorescent tags on diffusion and biological activity is still unclear. In this study, we experimentally determined the diffusion coefficient of gold nanoparticles using a label-free, optical tracking technique and evaluated the influence of protein concentration, charge and diameter on nanoparticle diffusion through biological media. We dispersed positively- and negatively-charged nanoparticles with diameters varying from 10 to 100 nm in a common cell culture media with different concentrations of serum proteins. Our results show that dynamic protein interactions influence nanoparticle diffusion in the range of serum concentrations tested. Experimental regimes to obtain quantitative information on the factors that influence the dynamics of nanoparticles in biological media have been developed.
摘要：
纳米技术是一个快速发展的领域，在生物应用中得到了广泛的研究。了解影响纳米粒子在生物流体中扩散的因素可以帮助开发各种技术。实时的发展,无标签跟踪技术将允许扩展纳米粒子扩散和活性的现有知识。基于荧光的显微镜是监测和跟踪纳米粒子动力学的最广泛的工具之一;然而，荧光标签对扩散和生物活性的影响尚不清楚。在这项研究中,我们通过实验确定了金纳米粒子的扩散系数，光学跟踪技术，并评估了蛋白质浓度的影响，纳米粒子通过生物介质扩散的电荷和直径。我们将直径从10到100nm不等的带正电荷和负电荷的纳米颗粒分散在具有不同血清蛋白浓度的普通细胞培养基中。我们的结果表明，动态蛋白质相互作用会影响纳米颗粒在测试的血清浓度范围内的扩散。已经开发了实验机制，以获得有关影响生物介质中纳米颗粒动力学的因素的定量信息。
