Abstract：
Pancreatic cancer is notoriously lethal due to its late diagnosis and poor patient response to treatments, posing a significant clinical challenge. This study introduced a novel approach that combines a single-cell capturing platform, tumor-targeted silver (Ag) nanoprobes, and precisely docking tapered fiber integrated with Raman spectroscopy. This approach focuses on early detection and progression monitoring of pancreatic cancer. Utilizing tumor-targeted Ag nanoparticles and tapered multimode fibers enhances Raman signals, minimizes light loss, and reduces background noise. This advanced Raman system allows for detailed molecular spectroscopic examination of individual cells, offering more practical information and enabling earlier detection and accurate staging of pancreatic cancer compared to conventional multicellular Raman spectroscopy. Transcriptomic analysis using high-throughput gene screening and transcriptomic databases confirmed the ability and accuracy of this method to identify molecular changes in normal, early, and metastatic pancreatic cancer cells. Key findings revealed that cell adhesion, migration, and the extracellular matrix are closely related to single-cell Raman spectroscopy (SCRS) results, highlighting components such as collagen, phospholipids, and carotene. Therefore, the SCRS approach provides a comprehensive view of the molecular composition, biological function, and material changes in cells, offering a novel, accurate, reliable, rapid, and efficient method for diagnosing and monitoring pancreatic cancer.
摘要：
众所周知，胰腺癌是致命的，因为它诊断晚，病人对治疗的反应差，构成了重大的临床挑战。这项研究引入了一种结合单细胞捕获平台的新方法，肿瘤靶向银（Ag）纳米探针，精确对接锥形光纤与拉曼光谱集成。这种方法侧重于胰腺癌的早期检测和进展监测。利用肿瘤靶向的Ag纳米颗粒和锥形多模纤维增强拉曼信号，最大限度地减少光损失，减少背景噪音。这种先进的拉曼系统可以对单个细胞进行详细的分子光谱检查，与传统的多细胞拉曼光谱相比，提供更多的实用信息，并能够更早地检测和准确分期胰腺癌。使用高通量基因筛选和转录组数据库的转录组分析证实了这种方法识别正常分子变化的能力和准确性，早期，和转移性胰腺癌细胞。关键发现揭示了细胞粘附，迁移,细胞外基质与单细胞拉曼光谱(SCRS)结果密切相关,突出胶原蛋白等成分，磷脂，和胡萝卜素。因此,SCRS方法提供了分子组成的全面视图，生物学功能,细胞中的物质变化，提供一本小说，准确,可靠,快速,诊断和监测胰腺癌的有效方法。
