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Abstract：
OBJECTIVE: Given the implications of concurrent human papilloma viral infection (HPV) in the prognostic course and implications on therapeutic approached of patients with oral squamous cell carcinoma (OSCC), we seek to investigate the implications that P16 expression has on the clinical course and pathological appearance of patients with OSCC and concurrent infection.
METHODS: Using S-P immunohistochemistry, we examined the expression of P16 and Ki67 in 460 patients with OSCC. We compared the expression of the protein between the tumor cells and normal epithelial mucosa within the same patient. The clinical and pathological characteristics (including gender, age, histological grade, lymph node metastasis, clinical stage, clinical recurrence, tumor diameter, Ki67 proliferation index) were analyzed by stratification statistically.
RESULTS: In total 460 cases of OSCC were identified and expression of P16 was significantly higher in the OSCC group compared to the normal mucosal epithelial group (X2 = 60.545, p = .000). There also appear to be a gender predilection as the expression was higher in females compared to males (0.218 vs. 0.144, X2 = 3.921, p = .048). Younger age also appears to be a predictive factor as those under 35 years old had higher expression of the protein compared to those over 35 years old (0.294 vs. 0.157, X2 = 4.230, p = .040). P16 positivity showed a significant positive correlation with histologic grade (X2 = 4.114, p = .043). In addition, the positive rate of P16 was higher in patients with ki67 over 85% (0.455 vs. 0.160, X2 = 6.667, p = .023).
CONCLUSIONS: OSCC with HPV infection tends to occur more frequently in female patients and those under 35 years of age. HPV infection with expression of the P16 and ki67 protein may promote the proliferation and growth of OSCC at a higher frequency.
METHODS: Using S-P immunohistochemistry, we examined the expression of P16 and Ki67 in 460 patients with OSCC. We compared the expression of the protein between the tumor cells and normal epithelial mucosa within the same patient. The clinical and pathological characteristics (including gender, age, histological grade, lymph node metastasis, clinical stage, clinical recurrence, tumor diameter, Ki67 proliferation index) were analyzed by stratification statistically.
RESULTS: In total 460 cases of OSCC were identified and expression of P16 was significantly higher in the OSCC group compared to the normal mucosal epithelial group (X2 = 60.545, p = .000). There also appear to be a gender predilection as the expression was higher in females compared to males (0.218 vs. 0.144, X2 = 3.921, p = .048). Younger age also appears to be a predictive factor as those under 35 years old had higher expression of the protein compared to those over 35 years old (0.294 vs. 0.157, X2 = 4.230, p = .040). P16 positivity showed a significant positive correlation with histologic grade (X2 = 4.114, p = .043). In addition, the positive rate of P16 was higher in patients with ki67 over 85% (0.455 vs. 0.160, X2 = 6.667, p = .023).
CONCLUSIONS: OSCC with HPV infection tends to occur more frequently in female patients and those under 35 years of age. HPV infection with expression of the P16 and ki67 protein may promote the proliferation and growth of OSCC at a higher frequency.
摘要：
目的：鉴于人类乳头状瘤病毒感染（HPV）在预后过程中的影响以及对口腔鳞状细胞癌（OSCC）患者的治疗方法的影响，我们试图研究P16表达对OSCC和并发感染患者的临床病程和病理表现的影响.
方法：使用S-P免疫组织化学，我们检测了460例OSCC患者中P16和Ki67的表达。我们比较了同一患者肿瘤细胞和正常上皮粘膜之间蛋白质的表达。临床和病理特征(包括性别、年龄,组织学分级,淋巴结转移,临床分期,临床复发,肿瘤直径,Ki67增殖指数)进行分层统计学分析。
结果：共发现460例OSCC，与正常粘膜上皮组相比，OSCC组P16的表达明显更高（X2=60.545，p=.000）。似乎也有性别倾向，因为女性的表达高于男性（0.218vs.0.144，X2=3.921，p=.048）。年轻的年龄似乎也是一个预测因素，因为35岁以下的人与35岁以上的人相比，该蛋白的表达更高（0.294vs.0.157，X2=4.230，p=.040）。P16阳性与组织学分级呈显著正相关(X2=4.114,p=.043)。此外,在ki67患者中，P16的阳性率高于85%(0.455vs.0.160，X2=6.667，p=0.023）。
结论：OSCC合并HPV感染倾向于在女性患者和35岁以下患者中更频繁发生。P16和ki67蛋白表达的HPV感染可能以更高的频率促进OSCC的增殖和生长。
方法：使用S-P免疫组织化学，我们检测了460例OSCC患者中P16和Ki67的表达。我们比较了同一患者肿瘤细胞和正常上皮粘膜之间蛋白质的表达。临床和病理特征(包括性别、年龄,组织学分级,淋巴结转移,临床分期,临床复发,肿瘤直径,Ki67增殖指数)进行分层统计学分析。
结果：共发现460例OSCC，与正常粘膜上皮组相比，OSCC组P16的表达明显更高（X2=60.545，p=.000）。似乎也有性别倾向，因为女性的表达高于男性（0.218vs.0.144，X2=3.921，p=.048）。年轻的年龄似乎也是一个预测因素，因为35岁以下的人与35岁以上的人相比，该蛋白的表达更高（0.294vs.0.157，X2=4.230，p=.040）。P16阳性与组织学分级呈显著正相关(X2=4.114,p=.043)。此外,在ki67患者中，P16的阳性率高于85%(0.455vs.0.160，X2=6.667，p=0.023）。
结论：OSCC合并HPV感染倾向于在女性患者和35岁以下患者中更频繁发生。P16和ki67蛋白表达的HPV感染可能以更高的频率促进OSCC的增殖和生长。
