Abstract：
BACKGROUND: Due to the ability of metal ions to cross the blood-brain barrier, there has been interest in analyzing cerebrospinal fluid (CSF) for trace element concentrations to investigate possible correlations with neurodegenerative diseases. In this study, Sarstedt polypropylene CSF collection tubes were analyzed to determine the contamination levels of aluminum, titanium, chromium, manganese, cobalt, nickel, molybdenum, gadolinium, vanadium, arsenic, cadmium, mercury, lead, thallium, selenium, copper, zinc, and iron.
METHODS: Sarstedt polypropylene CSF collection tubes from 2 separate lots (n = 10 per lot) were filled with a 2 mL aliquot of a CSF pool with known element concentrations. After 24 hours of leaching at room temperature, all 18 elements were analyzed via inductively coupled plasma mass spectrometry (ICP-MS). Results were subtracted from the initial pool concentration to determine contamination levels.
RESULTS: No detectable contamination above the assay limit of detection was found in 11 analytes. Molybdenum and selenium contamination was measured in all tubes, and aluminum, titanium, manganese, thallium, and zinc had minimal levels of sporadic detectable contamination in 25% or fewer of the tubes tested.
CONCLUSIONS: Sarstedt polypropylene CSF tubes are an acceptable collection tube for the analysis of most assessed metals in CSF.
METHODS: Sarstedt polypropylene CSF collection tubes from 2 separate lots (n = 10 per lot) were filled with a 2 mL aliquot of a CSF pool with known element concentrations. After 24 hours of leaching at room temperature, all 18 elements were analyzed via inductively coupled plasma mass spectrometry (ICP-MS). Results were subtracted from the initial pool concentration to determine contamination levels.
RESULTS: No detectable contamination above the assay limit of detection was found in 11 analytes. Molybdenum and selenium contamination was measured in all tubes, and aluminum, titanium, manganese, thallium, and zinc had minimal levels of sporadic detectable contamination in 25% or fewer of the tubes tested.
CONCLUSIONS: Sarstedt polypropylene CSF tubes are an acceptable collection tube for the analysis of most assessed metals in CSF.
摘要：
背景：由于金属离子穿过血脑屏障的能力，分析脑脊液(CSF)的微量元素浓度,以研究与神经退行性疾病的可能相关性,引起了人们的兴趣.在这项研究中,分析Sarstedt聚丙烯CSF收集管以确定铝的污染水平，钛，铬,锰，钴,镍,钼,钆,钒，砷,镉,水银,铅,铊,硒,铜，锌，和铁。
方法:将来自2个单独批次(每批n=10)的Sarstedt聚丙烯CSF收集管填充具有已知元素浓度的CSF池的2mL等分试样。在室温下浸出24小时后，所有18种元素均通过电感耦合等离子体质谱法(ICP-MS)进行分析.从初始池浓度中减去结果以确定污染水平。
结果：在11种分析物中没有发现超过检测极限的可检测污染。测量所有试管中的钼和硒污染，和铝，钛，锰，铊,在25%或更少的测试管中，锌的零星可检测污染水平最低。
结论：Sarstedt聚丙烯CSF管是分析CSF中大多数评估金属的可接受的收集管。
方法:将来自2个单独批次(每批n=10)的Sarstedt聚丙烯CSF收集管填充具有已知元素浓度的CSF池的2mL等分试样。在室温下浸出24小时后，所有18种元素均通过电感耦合等离子体质谱法(ICP-MS)进行分析.从初始池浓度中减去结果以确定污染水平。
结果：在11种分析物中没有发现超过检测极限的可检测污染。测量所有试管中的钼和硒污染，和铝，钛，锰，铊,在25%或更少的测试管中，锌的零星可检测污染水平最低。
结论：Sarstedt聚丙烯CSF管是分析CSF中大多数评估金属的可接受的收集管。
