PDF(Pubmed)
Abstract：
UNASSIGNED: Nuclear factor kappa (NF-κB) plays a key role in cancer cell proliferation; thus, small molecule inhibitors of NF-κB activity can effectively inhibit breast cancer (BC) progression. We have previously reported oxazine and piperazine-linked pyrimidines as novel anti-cancer agents that can suppress NF-κB activation in BC cells. Moreover, the TRX-01 compound, an oxazine-linked pyrimidine, inhibited MCF-7 cells at a concentration of 9.17 µM in the Alamar Blue assay.
UNASSIGNED: This work involved the analysis of frontier molecular orbitals, HOMO-LUMO interactions, and molecular electrostatic potential for the TRX-01 structure. Additionally, the TRX-01 compound was studied for cytotoxicity, and migration as well as invasion assays were performed on BC cells.
UNASSIGNED: Finally, TRX-01 blocked the translocation of NF-κB from the cytoplasm to the nucleus in MCF-7 cells and reduced NF-κB and IκBα levels in a dose-dependent manner. It also suppressed migratory and invasive properties of BC cells.
UNASSIGNED: Overall, the data indicates that TRX-01 can function as a novel blocker of BC growth and metastasis by targeting NF-κB activation.
UNASSIGNED: This work involved the analysis of frontier molecular orbitals, HOMO-LUMO interactions, and molecular electrostatic potential for the TRX-01 structure. Additionally, the TRX-01 compound was studied for cytotoxicity, and migration as well as invasion assays were performed on BC cells.
UNASSIGNED: Finally, TRX-01 blocked the translocation of NF-κB from the cytoplasm to the nucleus in MCF-7 cells and reduced NF-κB and IκBα levels in a dose-dependent manner. It also suppressed migratory and invasive properties of BC cells.
UNASSIGNED: Overall, the data indicates that TRX-01 can function as a novel blocker of BC growth and metastasis by targeting NF-κB activation.
摘要：
■核因子κB（NF-κB）在癌细胞增殖中起关键作用；因此，NF-κB活性的小分子抑制剂能有效抑制乳腺癌的进展。我们先前已经报道了恶嗪和哌嗪连接的嘧啶作为可以抑制BC细胞中NF-κB活化的新型抗癌剂。此外，TRX-01化合物,恶嗪连接的嘧啶,在AlamarBlue测定中，以9.17µM的浓度抑制MCF-7细胞。
■这项工作涉及对前沿分子轨道的分析，HOMO-LUMO相互作用,和TRX-01结构的分子静电势。此外,研究了TRX-01化合物的细胞毒性，在BC细胞上进行迁移和侵袭测定。
■最后，TRX-01在MCF-7细胞中阻断NF-κB从细胞质到细胞核的易位，并以剂量依赖的方式降低NF-κB和IκBα水平。它还抑制了BC细胞的迁移和侵袭特性。
■总的来说，数据表明,TRX-01可以通过靶向NF-κB激活而作为BC生长和转移的新型阻断剂发挥作用。
■这项工作涉及对前沿分子轨道的分析，HOMO-LUMO相互作用,和TRX-01结构的分子静电势。此外,研究了TRX-01化合物的细胞毒性，在BC细胞上进行迁移和侵袭测定。
■最后，TRX-01在MCF-7细胞中阻断NF-κB从细胞质到细胞核的易位，并以剂量依赖的方式降低NF-κB和IκBα水平。它还抑制了BC细胞的迁移和侵袭特性。
■总的来说，数据表明,TRX-01可以通过靶向NF-κB激活而作为BC生长和转移的新型阻断剂发挥作用。
