PDF(Pubmed)
Abstract：
Saiga antelope (Saiga tatarica) is a protected species in Kazakhstan. Little is known about the parasitofauna of these mammals. Therefore, the focus of this study was to evaluate the prevalence and species diversity of Eimeria spp. infection in the Volga-Ural Saiga antelope population. In June 2023, 104 Saiga antelope fecal samples collected from the district of Zhanibek, located in the province of West Kazakhstan were evaluated using microscopic and molecular techniques. Based on coprovoscopy results, Eimeria spp. Oocysts were present in 22 samples (21%). The four fecal samples containing the largest numbers of Eimeria spp. Oocysts per 10x field were selected for further genetic analysis. DNA extraction, nested PCR amplification, and sequencing were performed on 91 clones, with 80 clones forming a distinct clade and exhibiting genetic similarity to MT801034 Eimeria sp. Voucher HY3. These clones possibly represent an Eimeria specific to Saiga antelopes and gazelle that has previously been morphologically described as Eimeria elegans (Svanbaev, 1979), underscoring the importance of further research into parasitic infections in this protected species.
摘要：
赛加羚羊（Saigatatarica）是哈萨克斯坦的受保护物种。对这些哺乳动物的寄生虫知之甚少。因此,这项研究的重点是评估艾美球虫的患病率和物种多样性。伏尔加河-乌拉尔·赛加羚羊种群的感染。2023年6月，从Zhanibek地区收集了104个Saiga羚羊粪便样本，位于西哈萨克斯坦省，使用显微镜和分子技术进行了评估。根据共同的结果，艾美球虫。卵囊存在于22个样品中(21%)。四个粪便样品中含有最大数量的艾美球虫。选择每10x视野的卵囊用于进一步的遗传分析。DNA提取,巢式PCR扩增，对91个克隆进行测序，有80个克隆形成不同的进化枝，并表现出与MT801034艾美球虫的遗传相似性。凭证HY3。这些克隆可能代表了赛加羚羊和瞪羚特有的艾美球虫，以前在形态上被描述为艾美球虫（Svanbaev，1979),强调进一步研究这个受保护物种中寄生虫感染的重要性。
