Abstract：
The study of microtubules arrangements and dynamics during axon outgrowth and pathfinding has gained scientific interest during the last decade, and numerous technical resources for its visualization and analysis have been implemented. In this chapter, we describe the cell culture protocols of embryonic cortical and retinal neurons, the methods for transfecting them with fluorescent reporters of microtubule polymerization, and the procedures for time-lapse imaging and quantification in order to study microtubule dynamics during axon morphogenesis.
摘要：
在过去的十年中，轴突生长和寻路过程中微管排列和动力学的研究获得了科学兴趣。并且已经实现了用于其可视化和分析的大量技术资源。在这一章中,我们描述了胚胎皮质和视网膜神经元的细胞培养方案，用微管聚合的荧光报告基因转染它们的方法，以及延时成像和定量程序，以研究轴突形态发生过程中的微管动力学。
