PDF(Pubmed)
Abstract：
UNASSIGNED: Unlike plant phytochemicals, little has been done to explore the metabolites from phyllosphere bacterial flora, some of which enabled them to survive interspecific competition through amensalism. This study evaluated the antimicrobial activity of metabolites from Phyllospheric Bacteria (PB) isolated from Funtumia elastica (FE), against selected bacterial and fungal pathogens. Phenotypic and molecular methods were used to identify the isolated phyllo-microbiota.
UNASSIGNED: The PB were aseptically isolated by sonication. Their metabolites were obtained from the fresh overnight culture of the organisms. The cell-free supernatants containing the metabolites were used for antimicrobial assays against the pathogens. The DNA of the bacterial isolates were isolated using a NIMR-BIOTECH DNA extraction kit, while their 16S rRNA was amplified with the primer: 799F 5\'-AACACGGATTA GATACC-3\', 1193R 5\'- ACGTCATCCCCACCTTCC-3\', using SolisFast* Master Mix, (Solis Biodyne-Estonia). The BLAST of the sequence was done from the NCBI Gen-bank. The PB strains identified were submitted to NCBI and accession numbers were assigned to them.
UNASSIGNED: The phyllosphere of FE yielded 21 bacterial isolates: 7 Gram-positives and 14 Gram-negatives. The metabolites from these isolates showed varying degrees of bioactivity against Staphylococcus aureus (ATCC29213), Escherichia coli (ATCC 25922) Klebsiella pneumoniae (ATCC 35659); Trychophyton rubrum, Candida albicans and Microsporum canis. Fifteen bioactive isolates sequenced yielded four genera, Enterobacter (E. hormaechei 98.44%), Bacillus (B. cereus 100%), Pontoea (P. dispersa 99.72%), Staphylococcus (S. arlettae 99.72%).
UNASSIGNED: Bacteria from FE phyllosphere, produced metabolites antagonistic (cidal) to some human pathogens. This has great potential for possible drug discovery.
UNASSIGNED: The PB were aseptically isolated by sonication. Their metabolites were obtained from the fresh overnight culture of the organisms. The cell-free supernatants containing the metabolites were used for antimicrobial assays against the pathogens. The DNA of the bacterial isolates were isolated using a NIMR-BIOTECH DNA extraction kit, while their 16S rRNA was amplified with the primer: 799F 5\'-AACACGGATTA GATACC-3\', 1193R 5\'- ACGTCATCCCCACCTTCC-3\', using SolisFast* Master Mix, (Solis Biodyne-Estonia). The BLAST of the sequence was done from the NCBI Gen-bank. The PB strains identified were submitted to NCBI and accession numbers were assigned to them.
UNASSIGNED: The phyllosphere of FE yielded 21 bacterial isolates: 7 Gram-positives and 14 Gram-negatives. The metabolites from these isolates showed varying degrees of bioactivity against Staphylococcus aureus (ATCC29213), Escherichia coli (ATCC 25922) Klebsiella pneumoniae (ATCC 35659); Trychophyton rubrum, Candida albicans and Microsporum canis. Fifteen bioactive isolates sequenced yielded four genera, Enterobacter (E. hormaechei 98.44%), Bacillus (B. cereus 100%), Pontoea (P. dispersa 99.72%), Staphylococcus (S. arlettae 99.72%).
UNASSIGNED: Bacteria from FE phyllosphere, produced metabolites antagonistic (cidal) to some human pathogens. This has great potential for possible drug discovery.
摘要：
■与植物化学物质不同，几乎没有做任何研究来探索叶球细菌菌群的代谢物，其中一些使他们能够通过非均主义在种间竞争中生存。这项研究评估了从弹性真菌（FE）中分离出的毛圈细菌（PB）的代谢物的抗菌活性，针对选定的细菌和真菌病原体。表型和分子方法用于鉴定分离的叶型微生物群。
■通过超声处理无菌分离PB。它们的代谢物是从生物体的新鲜过夜培养物中获得的。含有代谢物的无细胞上清液用于针对病原体的抗微生物测定。使用NIMR-BIOTECHDNA提取试剂盒分离细菌分离物的DNA，而他们的16SrRNA用引物扩增：799F5'-AACACGGATTAGATACC-3'，1193R5\'-ACGTCATCCCCCCACCTTCC-3\',使用SolisFast*MasterMix,（SolisBiodyne-爱沙尼亚）。序列的BLAST从NCBI基因库中进行。将鉴定的PB菌株提交给NCBI，并为其分配登录号。
■FE的叶球产生了21个细菌分离株：7个革兰氏阳性和14个革兰氏阴性。这些分离物的代谢物对金黄色葡萄球菌（ATCC29213）具有不同程度的生物活性，大肠埃希菌(ATCC25922)肺炎克雷伯菌(ATCC35659),白色念珠菌和犬小孢子菌。测序的15个生物活性分离株产生了四个属，肠杆菌(E.hormaechei98.44%)，芽孢杆菌(B.蜡质100%)，Pontoea(P.分散99.72%)，葡萄球菌(S.Arlettae99.72%）。
■来自FE叶球的细菌，产生的代谢物拮抗（杀）一些人类病原体。这对于可能的药物发现具有巨大的潜力。
■通过超声处理无菌分离PB。它们的代谢物是从生物体的新鲜过夜培养物中获得的。含有代谢物的无细胞上清液用于针对病原体的抗微生物测定。使用NIMR-BIOTECHDNA提取试剂盒分离细菌分离物的DNA，而他们的16SrRNA用引物扩增：799F5'-AACACGGATTAGATACC-3'，1193R5\'-ACGTCATCCCCCCACCTTCC-3\',使用SolisFast*MasterMix,（SolisBiodyne-爱沙尼亚）。序列的BLAST从NCBI基因库中进行。将鉴定的PB菌株提交给NCBI，并为其分配登录号。
■FE的叶球产生了21个细菌分离株：7个革兰氏阳性和14个革兰氏阴性。这些分离物的代谢物对金黄色葡萄球菌（ATCC29213）具有不同程度的生物活性，大肠埃希菌(ATCC25922)肺炎克雷伯菌(ATCC35659),白色念珠菌和犬小孢子菌。测序的15个生物活性分离株产生了四个属，肠杆菌(E.hormaechei98.44%)，芽孢杆菌(B.蜡质100%)，Pontoea(P.分散99.72%)，葡萄球菌(S.Arlettae99.72%）。
■来自FE叶球的细菌，产生的代谢物拮抗（杀）一些人类病原体。这对于可能的药物发现具有巨大的潜力。
