PDF(Pubmed)
Abstract：
UNASSIGNED: Synthetic food dyes are being exponentially used in food products and scarce studies regarding their toxicities and safety raise concern. Erythrosine is one of the synthetic food dyes being used in jams, fig, pineapple marmalades, dairy products, soft drinks, pickles, relishes, smoked fish, cheese, ketchup, maraschino cherries and a variety of other foods.
UNASSIGNED: In this study the cyto-genotoxic effect of erythrosine was evaluated, using root meristematic cells of Allium cepa for the cellular and molecular alternations at concentrations 0.1, 0.25, 0.5 and 1 mg/mL.
UNASSIGNED: The results revealed a significant decrease of 57.81% in the mitotic index after 96 h at the 0.1 mg/mL concentration. In biochemical analysis, the malondialdehyde content increased significantly (5.47-fold), while proline content, catalase activity and superoxide dismutase activity decreased gradually in a concentration-dependent manner showing a maximum decrease of 78.11%, 64.68% and 61.73% respectively at the highest concentration after 96 h duration. The comet assay revealed increased DNA damage with increasing concentration and attenuated total reflectance- Fourier transform infrared spectroscopy (ATR-FTIR) analysis showed significant alterations in biomolecules as indicated by multivariate analysis, i.e. Principal Component Analysis (PCA). Furthermore, molecular docking demonstrated a strong binding energy (Gbest = -11.46 kcal/mol) and an inhibition constant (Ki) of 3.96 nM between erythrosine and the DNA minor groove.
UNASSIGNED: The present study\'s findings revealed the cytotoxic and genotoxic potential of erythrosine on A. cepa root cells. Further, the study also proposed the usefulness of A. cepa as a model system for studying the toxicity of food additives.
UNASSIGNED: In this study the cyto-genotoxic effect of erythrosine was evaluated, using root meristematic cells of Allium cepa for the cellular and molecular alternations at concentrations 0.1, 0.25, 0.5 and 1 mg/mL.
UNASSIGNED: The results revealed a significant decrease of 57.81% in the mitotic index after 96 h at the 0.1 mg/mL concentration. In biochemical analysis, the malondialdehyde content increased significantly (5.47-fold), while proline content, catalase activity and superoxide dismutase activity decreased gradually in a concentration-dependent manner showing a maximum decrease of 78.11%, 64.68% and 61.73% respectively at the highest concentration after 96 h duration. The comet assay revealed increased DNA damage with increasing concentration and attenuated total reflectance- Fourier transform infrared spectroscopy (ATR-FTIR) analysis showed significant alterations in biomolecules as indicated by multivariate analysis, i.e. Principal Component Analysis (PCA). Furthermore, molecular docking demonstrated a strong binding energy (Gbest = -11.46 kcal/mol) and an inhibition constant (Ki) of 3.96 nM between erythrosine and the DNA minor groove.
UNASSIGNED: The present study\'s findings revealed the cytotoxic and genotoxic potential of erythrosine on A. cepa root cells. Further, the study also proposed the usefulness of A. cepa as a model system for studying the toxicity of food additives.
摘要：
■合成食品染料在食品中呈指数级使用，有关其毒性和安全性的研究很少引起人们的关注。赤藓红是用于果酱的合成食品染料之一，无花果,菠萝果酱,乳制品,软饮料,泡菜,津津有味,熏鱼,奶酪,番茄酱,maraschino樱桃和各种其他食物。
■在这项研究中，评估了赤藓红的细胞遗传毒性作用，使用洋葱根分生组织细胞以0.1、0.25、0.5和1mg/mL的浓度进行细胞和分子交替。
■结果表明，在0.1mg/mL浓度下96小时后，有丝分裂指数显着降低了57.81％。在生化分析中，丙二醛含量显着增加（5.47倍），而脯氨酸含量，过氧化氢酶活性和超氧化物歧化酶活性以浓度依赖性方式逐渐下降，最大下降78.11%，96h后最高浓度分别为64.68％和61.73％。彗星分析显示，随着浓度的增加，DNA损伤增加，衰减的全反射-傅立叶变换红外光谱（ATR-FTIR）分析显示生物分子发生了显着变化，如多元分析所示。即主成分分析(PCA)。此外，分子对接显示出强结合能（Gbest=-11.46kcal/mol）和在赤藓和DNA小沟之间的抑制常数（Ki）为3.96nM。
■本研究的发现揭示了赤霉素对A.cepa根细胞的细胞毒性和遗传毒性潜力。Further,该研究还提出了A.cepa作为研究食品添加剂毒性的模型系统的有用性。
■在这项研究中，评估了赤藓红的细胞遗传毒性作用，使用洋葱根分生组织细胞以0.1、0.25、0.5和1mg/mL的浓度进行细胞和分子交替。
■结果表明，在0.1mg/mL浓度下96小时后，有丝分裂指数显着降低了57.81％。在生化分析中，丙二醛含量显着增加（5.47倍），而脯氨酸含量，过氧化氢酶活性和超氧化物歧化酶活性以浓度依赖性方式逐渐下降，最大下降78.11%，96h后最高浓度分别为64.68％和61.73％。彗星分析显示，随着浓度的增加，DNA损伤增加，衰减的全反射-傅立叶变换红外光谱（ATR-FTIR）分析显示生物分子发生了显着变化，如多元分析所示。即主成分分析(PCA)。此外，分子对接显示出强结合能（Gbest=-11.46kcal/mol）和在赤藓和DNA小沟之间的抑制常数（Ki）为3.96nM。
■本研究的发现揭示了赤霉素对A.cepa根细胞的细胞毒性和遗传毒性潜力。Further,该研究还提出了A.cepa作为研究食品添加剂毒性的模型系统的有用性。
