PDF(Pubmed)
Abstract：
The Vanilla genus is crucial for global production in food, perfume, and pharmaceutical industries. However, exploitation threatens some species, leading to extinction. Traditional communities use vanilla for medicinal purposes, and there are species like Vanilla chamissonis Klotzsch and Vanilla bahiana Hoehne with potential to occupy the market. For this, methanolic extraction of these two mentioned species was conducted alongside Vanilla planifolia. Analyzes of the cell viability, mutagenic and genotoxic potential were performed. In the Ames test, the assays were performed with concentrations from 0.5 and 5000 μg/ml and on five strains. Only Vanilla planifolia exhibited mutagenicity at the highest concentration in the TA98 strain. Viability tests were performed within a dose range of 0.05-5000 µg/ml and 24, 48, and 72-hour exposures. It was possible to observe a reduction in cell viability observed only at the highest concentration, for all three species and both cell types tested. Genotoxicity induction by the extracts was assessed at concentrations from 0.5 to 500 µg/ml through the cytokinesis-block micronucleus assay. No genotoxic damage or reduction in the Nucleus Division Index (NDI). The study found no mutagenicity, cytotoxicity, or genotoxicity in the species tested, indicating potential human use for food or pharmaceutical purposes.
摘要：
香草属对全球食品生产至关重要，香水,和制药行业。然而,剥削威胁着一些物种，导致灭绝。传统社区将香草用于医疗目的，还有一些物种，如VanillachamissonisKlotzsch和VanillabahianaHoehne，具有占领市场的潜力。为此，与Vanillaplanifolia一起进行了这两个上述物种的甲醇提取。分析细胞活力，进行诱变和基因毒性潜力。在艾姆斯测试中,在浓度为0.5和5000μg/ml的情况下对5个菌株进行测定。在TA98菌株中，只有香草在最高浓度下表现出诱变性。在0.05-5000µg/ml的剂量范围内以及24、48和72小时的暴露时间内进行生存能力测试。可以观察到仅在最高浓度下观察到的细胞活力降低。所有三种物种和两种细胞类型的测试。通过胞质分裂阻滞微核试验，以0.5至500µg/ml的浓度评估提取物的遗传毒性诱导。核分裂指数(NDI)无基因毒性损害或减少。这项研究没有发现诱变性,细胞毒性，或所测试物种的遗传毒性，表明人类可能用于食品或制药目的。
