PDF(Pubmed)
Abstract：
This study investigated the influence of supplementing with jack beans on jejunal morphology, cecal short-chain fatty acids production, gene expression both of pro- and anti-inflammatory cytokines and tight junctions. Four treatment groups including 288 Indian River chicks that were one day old were randomized at random. While the treatment groups received jack bean supplementation at levels of 5 %, 10 %, and 15 %, the control group (0 %) was given a basal diet. For 11-35 days, each treatment consisted of 8 pens with 9 birds each. Supplementing with jack beans significantly enhanced butyrate production (P < 0.001), while at 10 % supplementation did not differ from control. Villus height (VH) and the ratio (VH:CD) were significantly (P < 0.001) increased by dietary treatments, while villus width (VW) and crypt depth (CD) were significantly (P < 0.05) decreased. TLR-3, TNF-a, and IL-6 were all significantly (P < 0.001) increased by dietary supplementation. However, at 15 %, TLR-3 and IL-6 were same with control. IL-18 was significantly (P < 0.05) decreased at 15 %. IL-10 decreased significantly (P < 0.001), but at 10 % same with control. At 5 and 10 %, IL-13 increased significantly (P < 0.001), whereas dietary treatments decreased at 15 % compared to control. Although ZO1 decreased significantly (P < 0.001) and OLCN increased significantly (P < 0.001), both ZO1 and OCLN were not significantly different from the control at 15 %. Dietary treatments significantly (P < 0.001) increased CLDN1 but did not differ from the control at 10 %. JAM2 decreased significantly (P < 0.001) with dietary treatments. In conclusion, jack bean supplementation may increase broiler chicken performance and intestinal health due to butyrate production. It may affect intestinal morphology and integrity by upregulating a tight junction protein gene. Jack beans also impacted jejunum immune responses and inflammatory cytokine gene expression.
摘要：
这项研究调查了补充杰克豆对空肠形态的影响，盲肠短链脂肪酸生产，促炎和抗炎细胞因子和紧密连接的基因表达。随机随机分配四个治疗组，包括288只一天大的印度河小鸡。虽然治疗组接受了5%的杰克豆补充剂，10%,15%,对照组(0%)给予基础饮食。11-35天,每个治疗包括8个围栏，每个9只鸟。补充杰克豆显著提高了丁酸的产量（P<0.001），而在10％的补充与对照组没有差异。绒毛高度(VH)和比率(VH:CD)显著增加(P<0.001),绒毛宽度（VW）和隐窝深度（CD）显着降低（P<0.05）。TLR-3,TNF-a,通过饮食补充，IL-6均显着增加（P<0.001）。然而,15%，TLR-3和IL-6与对照相同。IL-18在15%时显著降低(P<0.05)。IL-10显著降低(P<0.001),但与对照的10%相同。5%和10%，IL-13显著升高(P<0.001),而饮食治疗与对照组相比下降了15%。尽管ZO1显著降低(P<0.001),OLCN显著升高(P<0.001),在15%时,ZO1和OCLN与对照没有显著差异.饮食处理显著(P<0.001)增加CLDN1,但与对照没有10%的差异。JAM2随着饮食处理显著降低(P<0.001)。总之,由于丁酸盐的产生，补充杰克豆可能会增加肉鸡的生产性能和肠道健康。它可能通过上调紧密连接蛋白基因来影响肠道形态和完整性。杰克豆还影响空肠免疫反应和炎性细胞因子基因表达。
