Abstract：
OBJECTIVE: This study compared the effectiveness of various cleaning approaches, including spray rinsing, repreparing with diamond burs, and using phosphoric acid or sodium hypochlorite alone or with polyphenols (resveratrol or myricetin), in removing blood contamination from the dentine after adhesive light-curing.
METHODS: The contact angles of the treated surfaces were measured and scanning electron microscopy/ energy dispersive X-ray spectroscopy observation was performed. The bond strength and nanoleakage were assessed, and in situ zymography was performed before and after aging. Interactions between matrix metalloproteinase (MMP)-9 and polyphenols were evaluated using molecular dynamics and rhMMP-9 inhibition analyses. The destruction of sodium hypochlorite on collagen and the resistance of polyphenols-treated dentine collagen to enzymolysis were evaluated using the hydroxyproline (HYP) assay. The effect of polyphenols on dentine collagen crosslinking was assessed by Fourier Transform Infrared Spectroscopy.
RESULTS: The repreparation group had the lowest contact angle compared to the other groups. The spray rinsing group had the lowest bond strength and highest amounts of nanoleakage. Cleaning with phosphoric acid or sodium hypochlorite alone removed the blood contaminants and parts of the adhesive; moreover, applying polyphenols further improved the bond strength and decreased nanoleakage and MMP activity after aging. Both polyphenols inhibited rhMMP-9 activity and promoted collagen crosslinking. Sodium hypochlorite showed the maximum HYP release when used alone, which was decreased after adding polyphenols.
CONCLUSIONS: Phosphoric acid or sodium hypochlorite cleaning can remove blood contamination from the dentine surface after adhesive curing, and the addition of polyphenols can improve the durability of dentine bonding.
METHODS: The contact angles of the treated surfaces were measured and scanning electron microscopy/ energy dispersive X-ray spectroscopy observation was performed. The bond strength and nanoleakage were assessed, and in situ zymography was performed before and after aging. Interactions between matrix metalloproteinase (MMP)-9 and polyphenols were evaluated using molecular dynamics and rhMMP-9 inhibition analyses. The destruction of sodium hypochlorite on collagen and the resistance of polyphenols-treated dentine collagen to enzymolysis were evaluated using the hydroxyproline (HYP) assay. The effect of polyphenols on dentine collagen crosslinking was assessed by Fourier Transform Infrared Spectroscopy.
RESULTS: The repreparation group had the lowest contact angle compared to the other groups. The spray rinsing group had the lowest bond strength and highest amounts of nanoleakage. Cleaning with phosphoric acid or sodium hypochlorite alone removed the blood contaminants and parts of the adhesive; moreover, applying polyphenols further improved the bond strength and decreased nanoleakage and MMP activity after aging. Both polyphenols inhibited rhMMP-9 activity and promoted collagen crosslinking. Sodium hypochlorite showed the maximum HYP release when used alone, which was decreased after adding polyphenols.
CONCLUSIONS: Phosphoric acid or sodium hypochlorite cleaning can remove blood contamination from the dentine surface after adhesive curing, and the addition of polyphenols can improve the durability of dentine bonding.
摘要：
目的：本研究比较了各种清洁方法的有效性，包括喷雾冲洗，用钻石毛刺重新准备，并单独使用磷酸或次氯酸钠或与多酚（白藜芦醇或杨梅素）一起使用，在粘合剂光固化后从牙本质中去除血液污染。
方法:测量处理表面的接触角并进行扫描电子显微镜/能量色散X射线光谱观察。评估了粘结强度和纳米孔隙度，老化前后进行原位酶谱。使用分子动力学和rhMMP-9抑制分析评估了基质金属蛋白酶（MMP）-9和多酚之间的相互作用。使用羟脯氨酸（HYP）测定法评估了次氯酸钠对胶原蛋白的破坏以及多酚处理的牙本质胶原蛋白对酶解的抗性。通过傅里叶变换红外光谱法评估多酚对牙本质胶原交联的影响。
结果：与其他组相比，再准备组的接触角最低。喷雾漂洗组具有最低的粘结强度和最高的纳米泄漏量。单独用磷酸或次氯酸钠清洗，去除血液污染物和粘合剂的部分；此外，应用多酚进一步提高了粘结强度，降低了老化后的纳米孔隙和MMP活性。两种多酚均抑制rhMMP-9活性并促进胶原交联。次氯酸钠单独使用时显示最大的HYP释放，添加多酚后下降。
结论：磷酸或次氯酸钠清洁可以去除粘合剂固化后牙本质表面的血液污染，多酚的加入可以提高牙本质结合的耐久性。
方法:测量处理表面的接触角并进行扫描电子显微镜/能量色散X射线光谱观察。评估了粘结强度和纳米孔隙度，老化前后进行原位酶谱。使用分子动力学和rhMMP-9抑制分析评估了基质金属蛋白酶（MMP）-9和多酚之间的相互作用。使用羟脯氨酸（HYP）测定法评估了次氯酸钠对胶原蛋白的破坏以及多酚处理的牙本质胶原蛋白对酶解的抗性。通过傅里叶变换红外光谱法评估多酚对牙本质胶原交联的影响。
结果：与其他组相比，再准备组的接触角最低。喷雾漂洗组具有最低的粘结强度和最高的纳米泄漏量。单独用磷酸或次氯酸钠清洗，去除血液污染物和粘合剂的部分；此外，应用多酚进一步提高了粘结强度，降低了老化后的纳米孔隙和MMP活性。两种多酚均抑制rhMMP-9活性并促进胶原交联。次氯酸钠单独使用时显示最大的HYP释放，添加多酚后下降。
结论：磷酸或次氯酸钠清洁可以去除粘合剂固化后牙本质表面的血液污染，多酚的加入可以提高牙本质结合的耐久性。
