Abstract：
Long-term and excessive use of tetracycline hydrochloride (TC) can lead to its accumulation in the environment, which can cause water contamination, bacterial resistance, and food safety problems. 2,6-Pyridine dicarboxylic acid (DPA) is a major biomarker of Bacillus anthracis spores, and its rapid and sensitive detection is of great significance for disease prevention and counter-terrorism. A bifunctional ratiometric fluorescent nanoprobe has been fabricated to detect DPA and TC. 3,5-dicarboxyphenylboronic acid (BOP) was intercalated into layered europium hydroxide (LEuH) by the ion-exchange method and exfoliated into nanosheets as a fluorescent nanoprobe (PNP). DPA and TC could significantly enhance the red fluorescence of Eu3+ through the antenna effect under different excitation wavelengths, while the fluorescence of BOP can be used as a reference based on the constant emission intensity, realizing ratiometric detection. A low limit of detection (LOD) for the target (DPA: 9.7 nM, TC: 21.9 nM) can be achieved. In addition, visual detection of DPA and TC was realized using color recognition software based on the obvious color changes. This is the first ratiometric fluorescent nanoprobe based on layered rare-earth hydroxide (LRH) for the detection of DPA and TC simultaneously, which opens new ideas in the design of multifunctional probes.
摘要：
长期过量使用盐酸四环素(TC)会导致其在环境中积累,会导致水污染，细菌抗性，和食品安全问题。2,6-吡啶二羧酸(DPA)是炭疽芽孢杆菌的主要生物标志物,其快速、灵敏的检测对疾病预防和反恐具有重要意义。已经制造了双功能比率荧光纳米探针来检测DPA和TC。通过离子交换方法将3,5-二羧基苯基硼酸（BOP）插入层状氢氧化铕（LEuH）中，并作为荧光纳米探针（PNP）剥离成纳米片。DPA和TC可以通过不同激发波长下的天线效应显著增强Eu3+的红色荧光,而BOP的荧光可以作为基于恒定发射强度的参考，实现比率检测。目标的低检测限（LOD）（DPA：9.7nM，TC:21.9nM)。此外,基于明显的颜色变化，利用颜色识别软件实现DPA和TC的视觉检测。这是第一个基于层状稀土氢氧化物（LRH）的比率荧光纳米探针，用于同时检测DPA和TC，这为多功能探针的设计开辟了新的思路。
