Abstract：
CONCLUSIONS: Supplying monochromatic blue LED light during the day, but not at night, promotes early coloration and improves anthocyanin accumulation in the skin of grape berries. Specific light spectra, such as blue light, are known to promote the biosynthesis and accumulation of anthocyanins in fruit skins. However, research is scarce on whether supplement of blue light during different periods of one day can differ in their effect. Here, we compared the consequences of supplying blue light during the day and night on the accumulation of anthocyanins in pigmented grapevine (Vitis vinifera) berries. Two treatments of supplemented monochromatic blue light were tested, with light emitting diodes (LED) disposed close to the fruit zone, irradiating between 8:00 and 18:00 (Dayblue) or between 20:00 and 6:00 (Nightblue). Under the Dayblue treatment, berry coloration was accelerated and total anthocyanins in berry skins increased faster than the control (CK) and also when compared to the Nightblue condition. In fact, total anthocyanin content was similar between CK and Nightblue. qRT-PCR analysis indicated that Dayblue slightly improved the relative expression of the anthocyanin-structural gene UFGT and its regulator MYBA1. Instead, the expression of the light-reception and -signaling related genes CRY, HY5, HYH, and COP1 rapidly increased under Dayblue. This study provides insights into the effect of supplementing monochromatic LED blue light during the different periods of one day, on anthocyanins accumulation in the berry skin.
摘要：
结论：白天提供单色蓝色LED灯，但不是在晚上，促进早期着色并改善葡萄浆果皮肤中的花色苷积累。特定的光谱，如蓝光，已知促进水果皮中花色苷的生物合成和积累。然而,关于在一天的不同时期补充蓝光的效果是否会有所不同的研究很少。这里,我们比较了白天和晚上提供蓝光对色素葡萄（葡萄）浆果中花色苷积累的影响。测试了两种补充单色蓝光的处理，发光二极管（LED）靠近水果区，在8:00至18:00（天蓝）或20:00至6:00（夜蓝）之间照射。在Dayblue待遇下,浆果着色加速，浆果皮中的总花色苷比对照（CK）增加得更快，与夜蓝条件相比也是如此。事实上,CK和夜蓝的总花色苷含量相似。qRT-PCR分析表明，Dayblue略微提高了花色苷结构基因UFGT及其调节因子MYBA1的相对表达。相反，光接收和信号相关基因CRY的表达，HY5,HYH,COP1在Dayblue下迅速增加。这项研究提供了在一天的不同时期补充单色LED蓝光的效果的见解，花青素在浆果皮中积累。
