Abstract：
Autotrophic denitrification technology has gained increasing attention in recent years owing to its effectiveness, economical, and environmentally friendly nature. However, the sluggish reaction rate has emerged as the primary impediment to its widespread application. Herein, a bio-enhanced autotrophic denitrification reactor with modified loofah sponge (LS) immobilized microorganisms was established to achieve efficient denitrification. Under autotrophic conditions, a nitrate removal efficiency of 59.55 % (0.642 mg/L/h) and a manganese removal efficiency of 86.48 % were achieved after bio-enhance, which increased by 20.92 % and 36.34 %. The bioreactor achieved optimal performance with denitrification and manganese removal efficiencies of 99.84 % (1.09 mg/L/h) and 91.88 %. ETSA and 3D-EEM analysis reveled manganese promoting electron transfer and metabolic activity of microorganisms. High-throughput sequencing results revealed as the increase of Mn(II) concentration, Cupriavidus became one of the dominant strains in the reactor. Prediction of metabolic functions results proved the great potential for Mn(II)-autotrophic denitrification of LS bioreactor.
摘要：
自养反硝化技术因其有效性近年来受到越来越多的关注,经济,和环保性质。然而,反应速率缓慢已成为其广泛应用的主要障碍。在这里，建立了改性丝瓜海绵（LS）固定化微生物的生物增强型自养反硝化反应器，以实现高效反硝化。在自养条件下,生物强化后的硝酸盐去除效率为59.55%(0.642mg/L/h),锰去除效率为86.48%,分别增长20.92%和36.34%。该生物反应器的反硝化和除锰效率分别为99.84％（1.09mg/L/h）和91.88％。ETSA和3D-EEM分析揭示了锰促进微生物电子转移和代谢活性。高通量测序结果表明,随着Mn(II)浓度的增加,Cupriavidus成为反应器中的优势菌株之一。代谢功能的预测结果证明了LS生物反应器Mn（II）自养反硝化的巨大潜力。
