Abstract：
This study presents new injectable hydrogels based on hyaluronic acid and collagen type II that mimic the polysaccharide-protein structure of natural cartilage. After collagen isolation from chicken sternal cartilage, tyramine-grafted hyaluronic acid and collagen type II (HA-Tyr and COL-II-Tyr) were synthesized. Hybrid hydrogels were prepared with different ratios of HA-Tyr/COL-II-Tyr using horseradish peroxidase and noncytotoxic concentrations of hydrogen peroxide to encapsulate human bone marrow-derived mesenchymal stromal cells (hBM-MSCs). The findings showed that a higher HA-Tyr content resulted in a higher storage modulus and a lower hydrogel shrinkage, resulting in hydrogel swelling. Incorporating COL-II-Tyr into HA-Tyr hydrogels induced a more favorable microenvironment for hBM-MSCs chondrogenic differentiation. Compared to HA-Tyr alone, the hybrid HA-Tyr/COL-II-Tyr hydrogel promoted enhanced chondrocyte adhesion, spreading, proliferation, and upregulation of cartilage-related gene expression. These results highlight the promising potential of injectable HA-Tyr/COL-II-Tyr hybrid hydrogels to deliver cells for cartilage regeneration.
摘要：
这项研究提出了基于透明质酸和II型胶原蛋白的新型可注射水凝胶，可模拟天然软骨的多糖-蛋白质结构。从鸡胸骨软骨中分离胶原蛋白后，合成了酪胺接枝的透明质酸和II型胶原蛋白（HA-Tyr和COL-II-Tyr）。使用辣根过氧化物酶和非细胞毒性浓度的过氧化氢以不同比例的HA-Tyr/COL-II-Tyr制备混合水凝胶，以包封人骨髓来源的间充质基质细胞（hBM-MSC）。研究结果表明，较高的HA-Tyr含量导致较高的储能模量和较低的水凝胶收缩率，导致水凝胶溶胀。将COL-II-Tyr掺入HA-Tyr水凝胶中诱导更有利的微环境用于hBM-MSC软骨形成分化。与单独的HA-Tyr相比，混合HA-Tyr/COL-II-Tyr水凝胶促进软骨细胞粘附增强，传播,扩散,和软骨相关基因表达的上调。这些结果突出了可注射HA-Tyr/COL-II-Tyr混合水凝胶递送细胞用于软骨再生的有希望的潜力。
