Abstract：
Adipose tissue immune cells are heterogeneous and dynamic, alter metabolism, and drive immune responses. Here, we present a protocol for assessment and characterization of murine adipose tissue immune cells using fluorescence-based flow cytometry and sorting into pure populations. We describe steps for isolation of the stromovascular fraction, antibody staining, and data collection by flow cytometry. We also discuss common issues and troubleshooting steps. For complete details on the use and execution of this protocol, please refer to Carey et al.1.
摘要：
脂肪组织免疫细胞具有异质性和动态性,改变新陈代谢，驱动免疫反应。这里,我们提出了一种使用基于荧光的流式细胞术和分选成纯群体的小鼠脂肪组织免疫细胞的评估和表征方案。我们描述了分离基质血管部分的步骤，抗体染色，并通过流式细胞术收集数据。我们还将讨论常见问题和故障排除步骤。有关此协议的使用和执行的完整详细信息，PleaserefertoCareyetal.1.
