Abstract：
The virus neutralization test (VNT) is a functional immunoassay which detects the presence and quantity of neutralizing antibodies. It is a highly sensitive and specific test. As with most neutralization assays, the EHDV VNT does not react with all virus-targeting antibodies, but specifically with those antibodies that bind to VP2, the outermost capsid structural protein of the virus. The interaction between VP2 and neutralizing antibodies can block EHDV cell binding, neutralizing its infectivity. The detection and quantification of neutralizing antibodies are indicative of how protected an animal is against reinfection. The EHD VNT can therefore be a useful tool to monitor the efficacy of a vaccination campaign. VP2 is also the main determinant of EHDV serotype specificity, and so EHDV-neutralizing antibodies which target VP2 are also serotype-specific. Throughdetecting and quantifying neutralizing antibodies, the VNT can discriminate the EHDV serotype responsible for an infection and provides insights into the time of infection. It is considered the gold standard test for identifying and quantifying antibodies against EHDV serotypes present in test serum samples. The assay is performed in vitro and is based on inhibition of virus infectivity in the presence of neutralizing antibodies. A neutralizing antibody titer is determined through the presence or absence of cytopathic effect in a cell monolayer. The VNT is a relatively inexpensive assay using standard laboratory equipment; however, to perform the assay, cell cultures, significant time, intensive labor, and technical skill are required.
摘要：
病毒中和测试（VNT）是一种功能免疫测定，可检测中和抗体的存在和数量。这是一个高度敏感和特定的测试。和大多数中和试验一样,EHDVVNT不与所有病毒靶向抗体反应，但特别是那些与病毒最外层衣壳结构蛋白VP2结合的抗体。VP2和中和抗体之间的相互作用可以阻断EHDV细胞结合，中和它的传染性。中和抗体的检测和定量指示动物如何受到保护以抵抗再感染。因此,EHDVNT可以是监测疫苗接种活动的功效的有用工具。VP2也是EHDV血清型特异性的主要决定因素,因此靶向VP2的EHDV中和抗体也是血清型特异性的。通过检测和定量中和抗体，VNT可以区分负责感染的EHDV血清型，并提供对感染时间的见解。它被认为是用于鉴定和定量针对测试血清样品中存在的EHDV血清型的抗体的黄金标准测试。该测定在体外进行并且基于在中和抗体存在下抑制病毒感染性。通过在细胞单层中存在或不存在致细胞病变效应来确定中和抗体滴度。VNT是使用标准实验室设备的相对便宜的测定;然而，为了进行检测，细胞培养,重要的时间,密集的劳动，和技术技能是必需的。
