Abstract：
Homologous recombination plays pivotal roles in physical attachments and genetic diversity. In the past, it was studied among individuals from different populations. However, only few gametes from individual could generate offspring, which limits its exploration in nature selection. In the last few years, preimplantation blastocysts based on trio SNP-chip data were available in individuals for preimplantation genetic testing (PGT). In this protocol, we demonstrate how to detect meiotic recombination events and construct the genetic map based on trio SNP-chip data, obtained from biopsied blastocysts and their related individuals in PGT cycles, which may allow better understanding of recombination events in nature selection.
摘要：
同源重组在物理附着和遗传多样性中起着关键作用。在过去,它是在来自不同人群的个体中进行研究的。然而,只有很少的个体配子可以产生后代，这限制了它在自然选择上的探索。在过去的几年里，基于三重SNP芯片数据的植入前胚泡可用于个体的植入前遗传学检测(PGT).在这个协议中,我们展示了如何检测减数分裂重组事件和构建基于三重SNP芯片数据的遗传图谱，从PGT周期的活检胚泡及其相关个体获得，这可以更好地理解自然选择中的重组事件。
