Abstract：
BACKGROUND: Vitamin E ( -tocopherol) and cholesterol are crucial components in cellular protection and physiological processes. Their uses in biological media face challenges due to their poor solubility and stability.
OBJECTIVE: The study investigated the complex interactions of these bioactive compounds in various encapsulation systems of cyclodextrin and liposome, as well as dispersion in PEG-6000, in an attempt to improve the viability, motility, and preservation of ovine sperm cells.
METHODS: The work explored the in vitro dissolution kinetics of vitamin E (d-tocopherol) and cholesterol using semi-empirical models.
RESULTS: The release profiles of VitE and Chl varied considerably, depending on the specific carrier systems. For liposome-loaded VitE and Chl, the Korsmeyer-Peppas model gave the best fit; for CD/VitE and CD/Chl, the Higuchi model provided the best fit, whereas for PEG-6000 dispersions (VitE and Chl) both the Higuchi and Korsmeyer-Peppas models demonstrated the excellent fit. All systems indicated a Fickian diffusion mechanism dictated by the concentration gradient. The delivery of VitE and Chl with CD, liposome and PEG dispersion significantly increased sperm mobility and motility. The effect on the VCL parameter was the greatest by liposome-loaded VitE and Chl, followed by CD encapsulation and PEG-6000 dispersion.
CONCLUSIONS: The dynamics of vitamin E and cholesterol within innovative delivery systems offers valuable insights into the development of advanced solutions in reproductive health, particularly on improving the viability, motility of refrigerated ovine sperm cells. Doi.org/10.54680/fr24510110712.
OBJECTIVE: The study investigated the complex interactions of these bioactive compounds in various encapsulation systems of cyclodextrin and liposome, as well as dispersion in PEG-6000, in an attempt to improve the viability, motility, and preservation of ovine sperm cells.
METHODS: The work explored the in vitro dissolution kinetics of vitamin E (d-tocopherol) and cholesterol using semi-empirical models.
RESULTS: The release profiles of VitE and Chl varied considerably, depending on the specific carrier systems. For liposome-loaded VitE and Chl, the Korsmeyer-Peppas model gave the best fit; for CD/VitE and CD/Chl, the Higuchi model provided the best fit, whereas for PEG-6000 dispersions (VitE and Chl) both the Higuchi and Korsmeyer-Peppas models demonstrated the excellent fit. All systems indicated a Fickian diffusion mechanism dictated by the concentration gradient. The delivery of VitE and Chl with CD, liposome and PEG dispersion significantly increased sperm mobility and motility. The effect on the VCL parameter was the greatest by liposome-loaded VitE and Chl, followed by CD encapsulation and PEG-6000 dispersion.
CONCLUSIONS: The dynamics of vitamin E and cholesterol within innovative delivery systems offers valuable insights into the development of advanced solutions in reproductive health, particularly on improving the viability, motility of refrigerated ovine sperm cells. Doi.org/10.54680/fr24510110712.
摘要：
背景：维生素E（-生育酚）和胆固醇是细胞保护和生理过程中的关键成分。由于它们的溶解性和稳定性差,它们在生物介质中的应用面临挑战。
目的：该研究调查了这些生物活性化合物在环糊精和脂质体的各种包封体系中的复杂相互作用，以及在PEG-6000中的分散，试图提高生存能力，运动性,和保存绵羊精子细胞。
方法：该工作使用半经验模型探索了维生素E（d-生育酚）和胆固醇的体外溶出动力学。
结果：VitE和Chl的释放曲线变化很大，取决于具体的承运人系统。对于脂质体负载的VitE和Chl，Korsmeyer-Peppas模型最适合；对于CD/VIE和CD/Chl，Higuchi模型提供了最适合的，而对于PEG-6000分散体（VitE和Chl），Higuchi和Korsmeyer-Peppas模型均表现出极好的拟合。所有系统均表明由浓度梯度决定的Fickian扩散机制。与CD一起交付VIE和Chl，脂质体和PEG分散显着增加精子的流动性和运动性。脂质体负载的VitE和Chl对VCL参数的影响最大，然后进行CD封装和PEG-6000分散。
结论：创新输送系统中维生素E和胆固醇的动态变化为生殖健康高级解决方案的开发提供了宝贵的见解。特别是在提高生存能力方面，冷藏绵羊精子细胞的运动性。Doi.org/10.54680/fr24510110712。
目的：该研究调查了这些生物活性化合物在环糊精和脂质体的各种包封体系中的复杂相互作用，以及在PEG-6000中的分散，试图提高生存能力，运动性,和保存绵羊精子细胞。
方法：该工作使用半经验模型探索了维生素E（d-生育酚）和胆固醇的体外溶出动力学。
结果：VitE和Chl的释放曲线变化很大，取决于具体的承运人系统。对于脂质体负载的VitE和Chl，Korsmeyer-Peppas模型最适合；对于CD/VIE和CD/Chl，Higuchi模型提供了最适合的，而对于PEG-6000分散体（VitE和Chl），Higuchi和Korsmeyer-Peppas模型均表现出极好的拟合。所有系统均表明由浓度梯度决定的Fickian扩散机制。与CD一起交付VIE和Chl，脂质体和PEG分散显着增加精子的流动性和运动性。脂质体负载的VitE和Chl对VCL参数的影响最大，然后进行CD封装和PEG-6000分散。
结论：创新输送系统中维生素E和胆固醇的动态变化为生殖健康高级解决方案的开发提供了宝贵的见解。特别是在提高生存能力方面，冷藏绵羊精子细胞的运动性。Doi.org/10.54680/fr24510110712。
